North Floridian Hospitality

North Floridian Hospitality

Intrigued in travelling to Florida? Take Into Consideration North Florida for the following trip location. Many of Florida’s most popular destinations are in the south, the north component of the state, prolonging from Pensacola to Jacksonville, uses vacationers lots of what they anticipate from Florida with a spin of Southern Charm.
Traveling southern to Ponte Vedra Beach, house of the Tournament Players Championship at Sawgrass. Golf fans will certainly locate a myriad of the most world-renowned as well as respected golf training courses while the family members will appreciate its attractive coastlines and also serene way of life. Adhering to the coast will certainly take site visitors to St. Augustine, the countries earliest city, as well as residence to fantastic purchasing as well as coastlines.
This is not the house of hand trees and also renowned coastlines, yet antique real-time oaks, stretching vineyard houses, and also freshwater springtimes. With coastlines within a 30-minute drive and also lots of lakes, there are lots of locations to play as well as with 2 state colleges in community there is lots of arts and also social occasions to delight in.
The Gulf Coast coastlines, understood as the Redneck Riviera, are renowned for white sand coastlines and also cozy waters. The emerald shade water prolongs from Pensacola Bay to Panama City, consisting of coastlines such as Gulf Breeze, Fort Walton Beach, Destin, Seaside, Perdido Key, Navarre, Sandestin, Grayton Beach, Santa Rosa Beach, and also Seagrove. Panama City is the Spring Break capitol and also was called “The Best Beach in America” by Conde Nast Traveler Magazine.
Lovely coastlines, all-natural elegance, first-rate golf and also tennis, southerly friendliness, exceptional trip services, and also cozy Florida sunlight make North Florida the best place for any type of getaway.

Traveling southern to Ponte Vedra Beach, residence of the Tournament Players Championship at Sawgrass. Complying with the coast will certainly take site visitors to St. Augustine, the countries earliest city, and also house to fantastic purchasing and also coastlines. With coastlines within a 30-minute drive and also lots of lakes, there are lots of locations to play as well as with 2 state colleges in community there is lots of arts and also social occasions to delight in. The Gulf Coast coastlines, understood as the Redneck Riviera, are renowned for white sand coastlines as well as cozy waters. The emerald shade water expands from Pensacola Bay to Panama City, consisting of coastlines such as Gulf Breeze, Fort Walton Beach, Destin, Seaside, Perdido Key, Navarre, Sandestin, Grayton Beach, Santa Rosa Beach, as well as Seagrove.

Research Degree Insiders is 10!

I’ve written quite a few looking-back posts over the last three years, as I hit 200 posts and then 250 posts. But I realised I’d missed my 10th anniversary, which is a reason for one more reflection post. I guess these are a bigger version of my done lists or my writing journals. Whatever they are, thank you for being part of the journey.

On 12 February 2013, a little academic advisor with dreams about writing a book one day, registered a free domain with WordPress and started writing down the advice she was giving in workshops and individual sessions. Mostly, I kept seeing the same issues over and over again, and I wanted an individual resource pack that I could easily share with students.

I used to do worksheets that I photocopied and handed out, and they were great. I am told there are still copies of copies of copies of those worksheets floating around, being handed on from student to student. Even then I knew that having my advice somewhere that people could easily share would be useful too.

I also saw my work as being somehow scrappy and outside the system—which, to be clear, it absolutely was. My blog was an open secret, but it wasn’t in any way approved by my workplace. I had idiosyncratic ideas, and I talked about feelings, and I used swear words, and so many metaphors. This is back in the days when all the blogging people were relatively small fry. The Thesis Whisperer was about 3 years old, and Inger Mewburn had not yet become the Director of Research Training at ANU (let alone become a professor). Now… I look around at my colleagues and co-authors, and I think maybe we are the system.

Writing Well and Being Well for your PhD and Beyond started life as a book proposal I wrote in 2014 based on an early version of the writing cycle (which I discussed in one of my first posts from March 2013). So I’ve spent a lot of time back in those first posts, about procrastination, perfectionism, feelings, bodies and metaphors. Of course, ten years is a lot of teaching, learning, writing and rewriting (some of those early posts got re-written as second editions back in 2017, and one even got a third edition in 2020). In the new book, I give links to these earlier versions as a way of showing my working, of drafting in public.

Writing Well and Being Well is going to be the fourth book that started life on this blog. How to Fix Your Academic Writing Trouble, Level Up your Essays and Your PhD Survival Guideall developed tools and advice I first explored on here, while also including a lot of material from me and my co-authors that was entirely new or were part of my workshops.

There is also a lot of the blog that hasn’t stood the test of time so well, or never caught on with readers. I have published over 280 posts, but the top posts are pretty consistent across time. People come to the blog for advice on concise writing , for taking Cornell Method notes , for advice on how long your sentences should be, and for tackling perfectionism, but also for advice on what logic or knowledge is or how to talk about your methodology. At the other end, a lot of posts get one or zero views in a year. I find this really helpful—it means I know what my audience is looking for, and when an idea immediately resonates. If I think something is important but didn’t really take off… then I know I need to try again, refining it until it works for people.

People used to find the blog pretty equally via Twitter and Facebook, then Facebook dwindled as a referrer, and now Twitter is joining it as a minor driver of traffic, so I guess the blog is in the middle of outliving the social web. This year, the top referrer is the Android WordPress App. Mostly, people have found my work via search engines, but I am pretty sure that’s because I’m on the first page of Google results if you need advice on writing more concisely. But thanks to the longstanding MVP blogger referrers, The Thesis Whisperer (though I see Inger is moving away from WordPress, end of an era TM), Raul Pacheco-Vega and Jo VanEvery—the admiration is mutual and I hope the traffic is too 🙂

I’m not sure I actually have any advice that anyone else should follow here. Sometimes I posted 14 times a year and sometimes I posted over 50 times. I’ve posted weekly, fortnightly, and whenever I felt like it. I’ve live blogged, I’ve written short snippets and long essays and so many listicles. I’ve been heavily multi-media, and I’ve stuck mostly to text. I had two guest posts in the entire history of the blog.

Maybe the most useful thing has been all the ways I’ve failed in public, or shown how long it takes for books to get written (a decade! a decade for the writing cycle book!), or left up all those posts that no-one reads—and simultaneously shown that the failures don’t mean you aren’t succeeding. I’ve published so many books recently that I’ve started to lose count of which book I’m on. Fail freely, and you will be freed from the shackles of failure. Failure is just part of the experiment. The point isn’t that we need to avoid failure , it’s that being terrible at something the first time you try it has zero (ZERO!!!!!) correlation to whether this is a thing you can finally master.

Whether you come for the quick writing tips or the messy background stories of how writing really gets done, welcome. I’m glad you are here.

Photo by David Ballew on Unsplash

Francisco Antonio Pacheco: Revolutionizing Leadership Practices and Inspiring Generations

Francisco Antonio Pacheco: An Exemplary Manager and Visionary Leader

Born in 1865, Francisco Antonio Pacheco made an indelible mark on the realm of management and leadership during his remarkable career. Through his innovative thinking, astute decision-making abilities, and unwavering commitment to excellence, Pacheco established himself as a trailblazer in the field of management theory.

Early Years and Education:
Francisco Antonio Pacheco was born into a humble family on June 12, 1865, in the charming town of Córdoba, Spain. From an early age, he displayed exceptional intelligence and a natural inclination for problem-solving. Recognizing his potential, Pacheco’s parents encouraged him to pursue higher education.

Pacheco embarked on his academic journey at the prestigious University of Salamanca. Here he immersed himself in a diverse range of subjects including economics, psychology, philosophy, and organizational behavior. His insatiable curiosity led him to delve deep into various historical case studies on successful leaders from different industries.

Career Beginnings:
After completing his education with honors in 1888 at the age of 23 years old, Francisco Antonio Pacheco quickly rose through the ranks within several prominent organizations across Spain. His charismatic personality combined with sharp managerial skills earned him recognition as one of the most promising young managers of his time.

Managerial Philosophy and Contributions:
Drawing upon insights gained from both academia and practical experience throughout his career, Francisco Antonio Pacheco developed a unique managerial philosophy based on humanistic principles. He firmly believed that every employee possessed untapped potential waiting to be harnessed for organizational success.

Pioneering ideas such as participative decision-making processes and fostering open communication channels within organizations became hallmarks of Pacheco’s management approach. He envisioned creating work environments where individuals felt empowered to contribute their ideas fearlessly while fostering collaboration among team members.

One of Pacheco’s most significant contributions to the field of management was his emphasis on continuous learning and development. He recognized that organizations thrived when employees constantly expanded their skill sets and knowledge. Pacheco introduced innovative training programs, mentoring initiatives, and encouraged his subordinates to pursue further education.

Unparalleled Success:
Francisco Antonio Pacheco’s visionary leadership and managerial acumen propelled him to unprecedented success throughout his career. Under his guidance, the organizations he managed experienced remarkable growth in terms of productivity, profitability, and employee satisfaction.

Pacheco’s relentless pursuit of excellence led him to win numerous accolades and recognition within the industry. He frequently presented papers at international conferences, sharing his valuable insights on effective management techniques with professionals worldwide.

Legacy:
Even after Francisco Antonio Pacheco’s passing in 1935 at the age of 70 years old, his ideas continue to shape modern management practices. His emphasis on fostering a positive work culture that nurtures talent has become an integral part of organizational structures across industries globally.

Today, countless managers draw inspiration from Pacheco’s groundbreaking ideas as they strive to create harmonious workplaces that drive innovation and maximize productivity. His legacy as a compassionate leader who put people at the center of every managerial decision remains a guiding star for future generations aspiring to excel in this ever-evolving field.

Thus, Francisco Antonio Pacheco will forever be remembered as an exemplary manager who revolutionized leadership practices through his visionary thinking, compassionate approach towards employees’ well-being, and unwavering commitment towards excellence.

Frontiers | The promotion of critical reading through the digital environment: A study on the virtual epitexts used to promote children’s picturebooks

1. Introduction

The coexistence of analog and digital paradigms in today’s information society has established a new reading ecosystem built on hybridization. This is evidenced in research into reading habits cited by scholars such as Cordón-García (2018, 2020) or Lluch (2018). The paradigm shift in reading has led to significant changes in the way children and young people approach information, knowledge and literature (Lluch, 2018). Social media have further influenced access to reading by throwing literate practices into an environment of constant and rapid modifications (Cruces, 2017; Lluch, 2017, 2018, 2021; Cordón-García, 2018). Contrary to expectations, the progress of digital publishing has strengthened the material aspect of reading to such an extent that the physicality of the book as an object has become one of the key elements in the new ecosystem, since, as Littau (2006) states, the format determines both the content and the layout.

In light of the challenges the digital society poses to reader training, the research conducted by Wolf (2018) is of particular interest. In her study, she defends the construction of a biliterate reading brain as the way we read influences the way we think. Wolf (2018) understands that the different digital experiences modern readers use—social media, games, interactive platforms—distance them from the in-depth reading connected to the development of critical thinking. The author, therefore, suggests a paradigm that combines analog and digital cultures to develop biliteracy from the beginning, with an emphasis on analog means in the early years to ensure access to the digital environment with guarantees at a later stage. The 2020 report by the Centro Regional para el Fomento del Libro en América Latina y el Caribe (Cerlalc) Lectura en papel vs. lectura en pantalla (Kovač and Van Der Weel, 2020) follows along the same lines. In one of its chapters, Støle (2020) develops the concept of in-depth reading by insisting on the conditions this entails, since reading on a screen, in her opinion, is inferior in terms of comprehension as digital media require less attention. In fact, as the Cerlalc Report (Kovač and Van Der Weel, 2020) indicates, difficulty in reading extensive texts rises as the number of personal digital devices available to children increases (Støle, 2020). This idea is further confirmed by the results of the report Developing Literacy Skills in a Digital World OECD (2021), presented by the OECD and based on the 2018 Pisa Report. Likewise, after reviewing 54 studies, Delgado et al. (2018) state that printed text is associated with better comprehension as paper reading requires greater concentration compared to screen reading (Gil-Pelluch et al., 2020). Furthermore, Schilhab et al. (2020) emphasize the sense of stability that printed paper bestows on a physical book, making it the most suitable medium for the development of in-depth reading. The European Commission report on the creation of a Work Plan for Culture (2015–2018), Promoting Reading in the Digital Environment (Unión Europea, 2016), and the report by the Federación del Gremio de Editores en España (Millán, 2017), among others, agree on this idea. Both reports provide robustness to this line of research, which emphasizes one of the challenges posed by the new ecosystem regarding the training of readers who are capable of selecting and organizing information to convert it into knowledge. In short, the digital society offers a new definition of the agents involved in the new reading ecosystem. Thus, the concept of the reader as prosumer (García-Roca and De-Amo, 2019; De Amo and García-Roca, 2021), the new role played by mediation (Lluch, 2017, 2021; Zafra, 2017; De Amo Sánchez-Fortún, 2021), reflection on the authorial paradigm (Unsworth, 2015; Tabernero-Sala, 2019; Tabernero-Sala et al., 2022) and the entity that virtual epitexts acquire in the promotion of reading (Lluch et al., 2015; Lluch, 2018) define the need to experiment with reading models that help to train critical readers who are able to cope with the tension between digital and analog cultures, as recommended in the studies by Baron (2015, 2021) and the exploratory research by Mizrachi et al. (2018) and Mangen et al. (2019), among others.

In this context, one of the challenges that the information society faces regarding reader training is none other than how to incorporate critical citizens into the new cultural ecosystem—citizens who possess the necessary strategies to express themselves using their own judgment in the midst of digital and analog paradigms (De Amo Sánchez-Fortún, 2021).

In the field of education, the reports by UNESCO insist on the need to develop critical thinking as a cornerstone in the construction of democratic, participatory societies capable of collectively meeting the challenges of the twenty-first century (Caro-Valverde, 2018). This is also expressed in the report Reimagining our Futures Together: A New Social Contract for Education—commissioned by UNESCO and prepared by the Comisión Internacional sobre los Futuros de la Educación, 2022. This report aims to promote lifelong education as a collective project, backed by the commitment to human rights, democratic participation and care for the planet. These objectives advocate for the questioning of absolute truths by citizens and, in doing so, they establish an education system that guarantees the access to accurate information as a basis for a commitment to truth.

In Spain, Organic Law 3/2020, of 29 December, which amends Organic Law 2/2006, of 3 May, on Education (España, Cortes Generales, 2020) expands on UNESCO indications and supports training that encourages critical thinking in primary and secondary education. Furthermore, Royal Decree 157/2022, of 1 March, which establishes the organization and minimum teaching requirements for primary education (España, Ministerio de Educación y Formación Profesional, 2022), introduces the concept of critical thinking linked to the training of competent, autonomous, critical readers who can understand and interpret multimodal texts that help them to meet the challenges of the modern information society. Media and information literacy is considered for this purpose, since its teaching focuses on information search strategies, acknowledgment of authorship, handling different documentary sources and the comparison, organization, critical evaluation and creative communication of information. In this context, the studies by Ennis (2011, 2018) and Kuhn (2018, 2019), among others, are of particular interest.

Ennis (2011, 2018) defines critical thinking as the reasonable, reflective thinking a person adopts regarding a subject on which they need to take some kind of decision. In this sense, citizens should be required to possess the abilities of a critical thinker, such as being well-informed, supporting beliefs on truths, justifying decisions and presenting reasons including points of view that differ from their own, while considering others’ feelings.

Kuhn (2018, 2019) understands argumentation as the axis around which critical thinking gravitates and as a fundamentally collective and contextualized social and cultural practice that should be reflected in the socio-educational context, as suggested by Guzmán-Cedillo and Flores-Macías (2020) in their review of 73 studies conducted between 2000 and 2016 in educational settings. In this regard, Bezanilla et al. (2019) have reviewed the main methodologies used to foster critical thinking and suggest that teachers in these settings should employ strategies such as posing questions, choosing activities with a real-life context, using different information sources, stimulating reflective dialogue, referring to visual displays and analyzing the arguments formulated by the community and research. Likewise, Witarsa, and Muhammad (2023) propose a learning model based on inquiry, discovery and problem-solving.

Children’s picturebooks are particularly appropriate for developing reading literacy in the early years, the stage when the analog paradigm should be prioritized, since, as the main research studies show (Wolf, 2018), in-depth reading, which is essential for developing critical thinking, is linked to physical books. From this perspective, an analysis of the evolution of the picturebook toward what is called ‘non-fiction’ (Grilli, 2020, 2021; Tabernero-Sala, 2022; Tabernero-Sala and Laliena, 2023) in reader training is required, as the non-fiction book occupies one of the most vital sectors in the publishing market (Jan, 2021) and has influenced the development of children’s books as a whole (Tabernero-Sala, 2022). In this respect, researchers are beginning to investigate the natural pairing of critical reading and the non-fiction picturebook (Trigo-Ibáñez et al., 2022). This type of children’s book is now the most interesting genre in children’s picturebooks, both nationally and internationally (Smith and Robertson, 2019; Dindelli, 2021; Jan, 2021), due to its artistic nature (Grilli, 2021); it has also become the reading model presented in fictional discourse aimed at children (Goga, 2020; Goga et al., 2021; Kümmerling-Meibauer and Meibauer, 2021).

In keeping with the line begun in the studies by Sander’s (2018) relating to the analysis of non-fiction picturebooks in connection to critical reading, focusing on the analysis of virtual epitexts as one of the strategies to promote reading is appropriate insomuch that we consider it important to analyze whether mediation fosters critical reading and, if applicable, which strategies the mediator should identify in order to recognize the promotional discourses that favor the development of the critical reader.

Social media have prompted the appearance of public, virtual epitexts that promote reading and have been generated by an inherent need for dissemination of publishing projects. Research studies such as those by Grøn (2014); Lluch et al. (2015), Basaraba (2016); Tabernero-Sala (2016b,2018), Tabernero-Sala and Calvo-Valios (2016), Ibarra-Rius and Ballester-Roca (2017); Rovira-Collado (2017), Dimova et al. (2018), Lebrun et al. (2018), Romero-Oliva et al. (2019, 2020, 2023), and Bilushchak et al. (2020) emphasize the entity acquired by virtual epitexts used in the promotion of reading, both in disseminating and in proposing reading models that they defend by identifying a new type of social reader who makes essential changes to the construction of discourse. The twenty-first century reader receives information on multimedia devices that involve multimodal discourse. Similarly to Gray (2010), Lluch et al. (2015) emphasize the need to reflect on the importance of these types of promotional epitexts that, far from being auxiliary, paratextual elements—in the sense of the definition of paratexts suggested by Genette (2004)—actually create the text, are part of its identity and determine the meaning that the reader assigns to it by proposing the reading strategy as a type of ‘textual consumption’ (Lluch et al., 2015). In earlier research works (Tabernero-Sala, 2016b), we analyzed in more detail the nature of the book trailer as one of the most significant virtual epitexts, both from the perspective of the promotion of reading and regarding its potential in training the readers of the twenty-first century, in such a way that it may even have its own artistic entity, in accordance with what Unsworth (2015) terms ‘multimodal literary narratives’. In some cases, it may, in fact, become a way of bringing interpretive rewriting closer to the reader (Tabernero-Sala, 2021). Therefore, analysis of virtual epitexts is vitally important to make a detailed study of the new methods of book promotion and how these methods—insofar as they create meaning and guide reading—determine the receiver the discourse requires. Thus, as a means of promoting reading and books—insofar as they create meaning—virtual epitexts offer the possibility of mediated reading that, in the case of children’s books, involves a reading model with links to the context in which it occurs, so much so that in previous studies (Tabernero-Sala et al., 2022) we have investigated the connection of the book trailer as an epitext for book promotion that defines a reading model in the case of non-fiction picturebooks. We understand that virtual epitexts may have changed in recent years, particularly since the COVID-19 health crisis, when, as several reports prove (Cencerrado Malmierca and Yuste Tuero, 2020; ERI-Lectura, 2020; Sánchez-Muñoz, 2022), reading habits changed and the use of virtual environments reached an unprecedented magnitude.

On this basis, we believed that an updated study on the virtual epitexts of children’s picturebooks would be of interest. Our initial proposal was to investigate and reflect on the new tendencies in the digital promotion of children’s books and to analyze to what extent, in the new cultural ecosystem, the promotion of reading in the area of children’s publishers shows a commitment to training critical citizens, in keeping with the UNESCO reports enshrined in different educational laws. Therefore, using the theoretical framework described and, in line with the studies by Gray (2010), we hereby present this study on the virtual epitexts used to promote children’s picturebooks, with a particular focus on the book trailer as the most widely used and firmly established tool for virtual promotion in the digital environment. The research concentrates on the following objectives:

Objective 1. To define the current tendencies of the virtual epitexts used in the promotion of children’s picturebooks.

Objective 2. To identify the construction strategies in virtual epitexts that are likely to encourage critical reading.

Objective 3. To select a corpus of examples of virtual epitexts as a resource for the promotion of reading and the training of critical readers in socio-educational contexts.

2. Materials and methods

To achieve the research objectives, we conducted a documentary study based on the content analysis of a sample of 836 pieces of digital audio-visual material. These audio-visual documents or videos were published by 45 publishing houses specializing in children’s literature from 1 January 2020—an important year due to the COVID-19 pandemic—to 31 December 2022. The main function of this digital content was to promote the reading of picturebooks aimed at children, so they are considered virtual epitexts of the books (Tabernero-Sala, 2016b).

The process of selecting the 45 publishing houses and the 836 virtual epitexts consisted of three stages:

• Firstly, 68 publishers were selected as being of interest for the study. The main sources consulted in this stage were the directory of Spanish publishers specializing in children’s and young people’s books published by the Federación de Gremios de Editores de España, n.d., the publishing houses belonging to Asociación Álbum, n.d. and the Biblioteca Nacional de España, n.d. of specialist publishers. For this initial selection, two essential assessment criteria were considered: publishing in Spanish and being well-known publishers in the area of children’s literature.

• The second stage consisted of a review of the digital promotion methods used by the publishers in 2020, 2021 and 2022. The selection of the years responded to the first objective of the study—to define the current tendencies in digital promotion—and we were also interested in observing how these tendencies have evolved since the beginning of the COVID-19 pandemic. Different studies (Cencerrado Malmierca and Yuste Tuero, 2020; Sánchez-Muñoz, 2022) have explored how the pandemic has hindered access to books due to the closure of bookshops and libraries during 2020 or to social distancing, which affected reading in classrooms. We decided to analyze how publishers developed digital promotion of children’s picturebooks at that time, since, as shown in Figure 1, the number of publications on YouTube and Vimeo in 2020 increased. As for the promotion methods, we confirmed that the main, most stable method, which acted as content repository, was YouTube (Tabernero-Sala et al., 2022), and, to a lesser extent, Vimeo. Furthermore, publishers generally ran promotion on different social media, such as Instagram or Facebook, due to the immediate nature of these media and their great capacity for dissemination. The publications uploaded onto these media, however, posed problems related to their registration, such as frequent repetitions within the same document and instability. At the same time, we also observed that videos previously hosted on YouTube and Vimeo were usually disseminated through these media, so the study eventually focused on these two platforms. In this way, the final selection of publishers was adjusted according to the presence of the publisher on YouTube or Vimeo between 2020 and 2022. Table 1 shows the quantitative data of the selection process and the adjustment of the publishers selected. The sample was reduced from 68 to 45 publishing houses as some of the 68 publishers initially selected did not have a YouTube or Vimeo channel, others that did have one had not used it in the period from 2020 to 2022. These were, primarily, small, independent publishers, representing projects run by one or two people, usually involving a more limited capacity for media outreach. Additionally, certain large publishing houses had, in fact, used these channels but they had no audio-visual content aimed at promoting children’s picturebooks; in other words, some of the publishers selected had also published books for adult readers, so, in some cases, their promotional videos focused solely on this sector of the population, and consequently, they were excluded from the selection process if they did not have any promotional videos for children’s books. Table 2 presents the 45 publishing houses that were finally included in the research.

FIGURE 1

Figure 1. Audio-visual documents published on YouTube or Vimeo by publishers.

TABLE 1

Table 1. Process of selecting publishers.

TABLE 2

Table 2. Publishers in the study sample.

• The third stage of the document selection process consisted of a review of all the audio-visual publications on the official YouTube and Vimeo channels belonging to the 45 publishing houses—3,358 documents in total. This review identified the virtual epitexts used in the promotion of children’s picturebooks. Through this process, other types of content by some publishing houses were dismissed. The videos that were discarded from the study were those that promoted textbooks intended for the context of formal education or books aimed at young adult or adult audiences; we also discarded videos that presented pedagogical and educational conferences or videos that promoted other merchandising products, such as toys or videogames. In turn, videos aimed solely at the promotion of children’s picturebooks, whether they were works of fiction or non-fiction, were included in the selection, excluding textbooks; we selected book trailers with a cinematographic style, videos displaying the book as an object that only showed the book or part of the book, videos in which writers, editors, book-sellers, librarians, readers or other mediators presented, commented on or read the book, or videos that showed activities or the publishers celebrating an event about this type of book (Figure 2); in other words, this study included audio-visual content aimed at promoting children’s books, although we found that the promotional styles and the strategies used were varied and they merged together, thus revealing some significant tendencies. The analysis of this aspect was of interest to this study and the results are presented below. Lastly, considering these criteria, 836 audio-visual documents were selected for study. Table 3 lists the audio-visual documents published each year by the group of 45 publishers and the number of virtual epitexts that were selected for the study.

FIGURE 2

Figure 2. Type of videos for the promotion of children’s picturebooks selected, by publisher.

TABLE 3

Table 3. Audio-visual documents reviewed and documents selected.

Once the selection process had been completed, each publisher’s digital channel and the videos published were systematically registered arranged by date. This stage involved the registration of the quantitative data of interest for the research objectives and for the content analysis (Krippendorff, 2019). These data included the date of creation of the channel, the number of subscribers, the number of total views on the channel, the date each video was uploaded to the internet, the duration of each video, the number of views up to the date of the analysis and the corresponding links. Table 4 shows the registration sheet used for each of the 45 publishers.

TABLE 4

Table 4. Register sheet for audio-visual documents.

Following the registration of the audio-visual documents, a mainly qualitative content analysis was conducted focusing on the constructive and rhetorical strategies in the audio-visual discourse typical of digital book promotion (Basaraba, 2016). The content analysis was based on a process of categorization, coding, analysis, refining of categories and qualitative interpretation, using a narrative approach based on the postulates of the school of critical theory, as indicated by Ruiz-Olabuénaga (2009). The results of the analysis according to the research objectives are presented below.

3. Results

3.1. Current tendencies in digital promotion

As regards the first objective, the analysis of the audio-visual documents detected five emergent macrocategories. These macrocategories identify the most frequent components currently emphasized by the virtual epitexts analyzed and help to classify the videos according to their constructive model and to determine the main tendencies in digital promotion of children’s picturebooks. The macrocategories detected are listed and described in Table 5.

TABLE 5

Table 5. Emergent macrocategories.

Figure 3 presents, in absolute values, the incidence of these macrocategories in the virtual epitexts analyzed. The macrocategories are not mutually exclusive, as several may be present in the same video.

FIGURE 3

Figure 3. Macrocategories detected in the virtual epitexts.

3.1.1. Emphasis on the materiality of the book

Virtual epitexts that affect the materiality of the book is as a dominant tendency on some publishing channels. This type of video presents the book as an object, with an emphasis on its paratexts, mainly illustrations or other elements such as book covers, foldouts and pop-up components. Therefore, the picturebook aimed at children tends to be displayed as a promotional strategy, with an emphasis on its physical features, in contrast to other genres, such as the novel, predominantly focused on protecting the work. Promotion is based, therefore, on encouraging child readers to want to interact with the book and enjoy touching it, handling it and discovering what is hidden in its pages. The study identifies publishers that base promotion on a display of the book, such as Akiara Books, Apila, Edelvives and Zahorí Books. Other publishers have opted for this form of promotion at specific times, as was the case with Andana in 2021 or Penguin España in 2022.

Among the virtual epitexts that focus on the book as an object, differences can also be observed regarding their construction strategies. In this respect, videos usually concentrate on the turning of the pages: as the fast-motion turning of all the pages in the book employed by Apila or Nuevo Nueve; the leisurely page-turning used by Zahorí Books, Andana or some of the videos by Maeva or Tecolote; the page-turning that pauses to explore and handle the book, with zoom effects to emphasize the paratexts or certain content, such as in the videos by Edelvives; or some less frequent artistic approaches, such as some examples by A buen paso or Kókinos. Other publishers attempt to develop their own style, such as the recent publications by Penguin España, very short videos that present the book and some of its pages in a clearly edited format or the virtual epitexts from Akiara Books, which are contextualized in a natural environment and carefully edited as far as esthetics and the senses are concerned (Table 6).

TABLE 6

Table 6. Examples of virtual epitexts based on the book’s materiality.

3.1.2. Emphasis on video editing and the esthetic component

Animated book trailers, characterized by their cinematographic style (Tabernero-Sala, 2016b) and their emphasis on the esthetic component are among the virtual epitexts analyzed. The most outstanding examples stand out for replacing the still image, typical of a book, with a moving image, typical of the cinema, including a soundtrack and creating a story with its own artistic entity. These elements are usually linked to a storyline synopsis and address the reader directly through questions about the continuity of the story. These resources aim to create an intriguing context, arouse curiosity and thereby encourage reading of the book. These videos require careful, professional design and editing, which involves financial investment. Therefore, few publishing houses maintain the quality in this type of digital promotion, as it is increasingly being replaced by ‘homemade’ videos and live recordings without any editing. Publishers still opting for book trailers include A Fin de Cuentos, Kalandraka, Libre Albedrío and Libros del Zorro Rojo, whose videos are defined by their artistic quality. Other publishers such as Amanuta also used to publish professional animated book trailers, but have almost ceased to do so or use them exceptionally for certain outstanding works, as this type of video is not usually the main focus of their digital channel. This is the case of Akiara Books, Combel, Edelvives, or Zahorí Books. As for their duration, they last one minute on average, although there was a general tendency in 2022 to reduce the length of these videos—except for a few significant exceptions—possibly due to their dissemination on social media such as Instagram—where speed and brevity are the priority—and to their cost. Table 7 presents some animated and cinematic-style book trailers.

TABLE 7

Table 7. Examples of animated and cinematic-style book trailers.

3.1.3. Emphasis on the interpretation or construction process of the book

The presence of authors and editors in virtual publications is a tendency shared Most of the publishers’ channels analyzed. The reason for their appearance may be to read the book, as we will see but, most usually, they appear to present books, promote reading, provide an interpretive commentary or reflect on the creative process. There are several different formats of virtual epitexts that emphasize these aspects according to, above all, the degree of complexity of the editing. On one hand, there are extensive formats, such as interviews, conferences and events. These videos are frequently recorded live with the participation of different guests or they even offer open access to the audience. Solo recordings of the authors commenting on their work are also common. The channels of the publishing houses A buen paso, Babel Libros, Combel, Diego Pun, Ediciones El Naranjo, Fondo de Cultura Económica, Nórdica or Wonder Ponder, among others, contain this type of publication, mainly intended for adult mediators, although content aimed at children—such as some videos by Combel—can also be found. The publisher Takatuka launched the initiative ‘Las librerías recomiendan,’ a series of videos in which different booksellers recommend and comment on a book by this publisher. The frequency of these publications is remarkable, once again, in 2020. They commonly contain references to the lockdown and the benefits of reading in this context. On the other hand, more elaborate formats requiring editing are also published, showing the authors’ workspaces or following the development of their creative process. This type of video is less common but publishers such as Andana, Edelvives, Pequeño Editor, Penguin España or Takatuka have some noteworthy titles (Table 8).

TABLE 8

Table 8. Examples of virtual epitexts focusing on understanding and the creative process.

3.1.4. Emphasis on the reading of the book

A marked tendency, mainly in 2020, is that of digital publications aimed at the reading of the book. In them, authors, editors, youtubers and other adult mediators read, sing or tell a story. A significant example is the ‘cuentacuentos’ (storyteller) initiative by the publisher Juventud, with 32 publications between April and July 2020. In these videos, the pages are displayed while a voiceover reads the text. Other publishing houses take the same line. For example, Carambuco provides publications both in an oral format and in sign language while displaying animated scenes from the book. In 2020 as well, Combel launched ‘cuentacombel,’ a project that offered, among others, readings of chapters of Las aventuras de Pinocho with the participation of different writers. Publishing houses such as Lóguez, Océano Travesía, Anaya, Fondo de Cultura Económica or Pintar-Pintar issued publications with the same purpose. On many occasions, the authors of the works or other mediators record the reading at home, as occurred during the lockdown. These initiatives did not continue after 2020, but they helped to boost a type of promotional video—characterized by its spontaneity—that has been used since then by many of the publishers analyzed. Furthermore, there are also edited virtual epitexts, of a more artistic nature, in which animation or the contemplation of the book are complemented by a voice telling part of the story, as is the case in some of the videos by Kalandraka or Ediciones Castillo. Table 9 provides examples of formats that emphasize the reading of the book.

TABLE 9

Table 9. Examples of virtual epitexts focusing on the reading of the book.

3.1.5. Emphasis on the reception of the book and its receiver

Another emerging tendency, a minority in quantitative terms but still of interest to this study, is the presence of child mediators who read, give opinions, recommend or interact with the books. In these videos, the publisher’s promotion relies on the receivers and peer dialogue, by trying to arouse empathy in the child audience. Similarly, some videos point out how to use a book, contain activities linked to the work or show its ludic and creative possibilities apart from reading it, even referring to other virtual epitexts, such as interactive games. These virtual epitexts emphasize the reception process of the book and offer replicable models for child readers; in other words, they provide ideas and proposals that extend the reading of the work and boost its potentiality. Again, there are two types among the publications showing this tendency. On one hand, there are more spontaneous videos, which consist of everyday recordings intended for immediate dissemination, for instance, some publications by Fondo de Cultura Económica or the publisher Pintar-Pintar, or the initiative by Wonder Ponder designed in 2020, which encourages children to participate by explaining how they felt during lockdown. On the other hand, although less frequently, there are more elaborate videos in which promotion is the underlying purpose. Their construction involves design, editing, animation and attention to the esthetics of the virtual epitext, as in some significant examples by the publishers Kalandraka or Zahorí Books (Table 10).

TABLE 10

Table 10. Examples of virtual epitexts that emphasize the role of the child reader and extend the reception process.

3.2. Strategies for promoting critical reading

After exploring the current tendencies that define the virtual epitexts selected, the analysis then focused on identifying the discourse markers and strategies likely to encourage critical reading, while also considering the theoretical framework established previously. To this effect, a selective analysis of a qualitative and interpretive nature was conducted with the purpose of fulfilling study objectives 2 and 3; in other words, in this case, the interest of the study lay not so much in the frequency with which a specific discourse marker appeared in the virtual epitexts, but rather in identifying, in the wide range of publications previously described, the discourse markers present in the virtual epitexts most likely to promote critical reading. To this effect, we separated and analyzed the discourse markers that identified strategies that promoted a type of reading focused on cracks in the discourse, on questioning the sources and developing empathy as a method to raise awareness resulting from access to knowledge (Ennis, 2011, 2018; Kuhn, 2018, 2019; Bezanilla et al., 2019). The analysis was complemented by a selection of virtual epitexts with comments, which are suggested, ultimately, as resources that may be of interest to mediators and readers in promoting reading and training critical readers in socio-educational contexts. The virtual epitexts in the final selection promote books that should also be recommended for classroom reading in order to favor the development of biliterate critical reading by merging analog and digital formats (Wolf, 2018). Table 11 shows the discourse markers and strategies identified by this second analysis that are subsequently commented and exemplified.

TABLE 11

Table 11. Discourse markers and strategies identified.

3.2.1. The visible author as a metadiscursive strategy

As confirmed by the tendency analysis, one of the recurring markers in virtual epitexts used for the promotion of children’s books is the presence of the author. Documents containing examples of this discourse marker can be found in promotions such as those by the publisher A buen paso for Semillas. Un Pequeño gran viaje by Alonso and Paschetta (2018) and Zum zum. El viaje de la semilla by Ferrada and Paschetta (2021). In this case, it involves a conversation between the editor and the illustrator of the two works regarding the creative processes.1 This provides an explanation about the universe in which the illustrator works according to the publisher’s proposal. Similarly, Jeffers (2019) appears in the book trailer for El destino de Fausto, a classic-style book trailer with sophisticated animation.2 Along the same lines, Pacheco (2020) explains his interpretation process regarding the work by Sister Juana Inés de la Cruz in Caperucita Roja, Primero sueño,3 Goes (2016) develops his creative activity4 or Dautremer (2021) demonstrates her creative process in her workspace, without saying a word, in Tan solo un instante.5 In these cases, the visibility of the people behind the books increases, which leads to the personalization of a discourse that does not come from the anonymity typical of children’s books, but rather from a discourse with its own ideology that corresponds to the author’s ‘world view.’ Therefore, whether fictional or non-fictional, the discourse is presented as a subjective proposal that responds to the bias of the person who has created it and that may, in this way, be questioned by the reader, who is required to form an opinion. More specifically, certain epitexts emerge as paradigmatic, such as those related to the recent promotion of two particularly relevant picturebooks: Dos ositos by Ylla (2021) and Ernesto el elefante by Browne (2021). The virtual promotion document of Dos ositos6 follows the style of a traditional book trailer in the first part—using a narrative voiceover with moving images of the illustrations in the foreground, which are photographs of the protagonists—and, the second, shorter part presents the author, her particular features, her life plan and her image contextualized in the time the work was created. Thus, the reader and mediator are presented with the directions the dialogue may subsequently take in the work, awareness of sustainable development and love of animals and the use of documentary images, which ensures the hybridization of reality and fiction. To a lesser extent, the promotional book trailer of Ernesto el elefante,7 incorporates the figure of the author to contextualize the proposal and suggest contemplation of the work within the framework of an entire authorial universe with an ideological bias.

On these lines, we recover a study by Sander’s (2018) on the connection of the non-fiction book with critical reading, since we understand that his research may apply, to some extent, to the analysis of children’s books, whether fictional or not, in a critical sense. In his study A literature of questions. Non-fiction for the Critical Child, Sander’s (2018) defines the genre as a literature of questions rather than answers requiring a critical reader to construct its meaning.

Sanders analyses the non-fiction book in terms of the presentation of information, of the bias authors place on the book simply by selecting some pieces of information to the detriment of others; he also investigates the esthetic strategies that invite readers to become involved in the texts, to interact with them, to engage with the information in a reflective, dialogic way. The intention is not to present information for it to be merely absorbed, but rather to encourage readers to investigate for themselves by arousing their curiosity and wonder in relation to what is presented.

These works propose critical readers who call into question what is presented to them, have an opinion on what they are shown, notice cracks in the discourse and question the credibility of the information. Based on ideas Barthe’s (1994) on the ‘death of the author’ and Freir’s (2002); Freire (2010) premises regarding critical pedagogy, along with Bakhtin’s (1981) dialogic concept of the novel, Sander’s (2018) identifies the critical reader in works with cracks in the authority of the text and questions that guide the process of intellectual inquiry. Therefore, centralization of meaning in a single, authorized source of true knowledge is questioned. Using the markers that Sanders identifies as the ‘visible author,’ the discourse presents the story using the voice, attitude and viewpoint of the author, who becomes part of the story through their ideas and opinions. Although, rhetorically, the interpretation may seem to be conditioned, Sanders bases his assertions on the research by Paxton and Zarnowski (Sander’s, 2018, p. 58), who showed, at the time, that readers’ interactive responses were more likely to occur when the author was present.

3.2.2. Empathic projection of the author on the discourse

Along the same lines, with an emphasis on metadiscursive visibility, book presentation from an emphatic perspective acquires particular relevance. In this way, readers identify themselves through exposure to the authors’ own experiences, whether they are writers or illustrators. This is the case, for example, in the promotional epitext for El patito feo (Andersen and Abramović, 2021), in which the illustrator, Marina Abramóvic, describes her experiential process of reading Andersen’s (2021) work8 or the promotional epitext for Muñeco de barro, in which the illustrator, Carme Solé-Vendrell (Reyes and Solé-Vendrell, 2020), talks about her personal interpretation of the characters and their surroundings.9 Also relating to awareness-raising, the editor of Libros del Zorro Rojo discusses Hablo como el río by Scott and Smith (2021), suggesting the lines of dialogue the story opens.10

3.2.3. Experiential reading: Between reality and fiction

In recent years, promotional virtual epitexts that defend removing the boundaries between fiction and non-fiction have taken the same direction. The intention is, ultimately, to relate reading to the reader’s personal experience and environment. Therefore, they show documents that encourage an emotional and physical connection between the books and the contents proposed. Thus, for example, in cultural and ideological terms, book trailers contain very clearly identified spaces and music. The book trailer for ¡ Gracias, madre Tierra!, illustrated by Starkoff (2022), based on the Haudenosaunee Thanksgiving Address is a clear example.11 It is the translation, in foldout format, of a remarkable prayer offering a greeting and gratitude to all living beings, which has been recited every morning for many centuries by the whole community in a territory located between the United States and Canada. This defines the book as an inventory of the natural world and the wonders that nature offers. The natural backdrop against which the work unfolds establishes a relationship between the book and its physical surroundings by reflecting on fictional and artistic discourses and their natural extension along the lines proposed by the Sustainable Development Goals contained in the 2030 Agenda and the report Reimagining our Futures Together: A New Social Contract for Education, prepared by the Comisión Internacional sobre los Futuros de la Educación, 2022. The book trailer for Tiempo de haikus by Santaeulàlia and Lozano (2022)12 contains the same theme and promotional concept.

3.2.4. The author, the reader, the book and the environment

In the register of experiential reading, there are promotional proposals that address the reader through questions raised directly by the works and generate interpretations of the setting by concealing the boundaries between the book and reality through the use of real scenarios where the act of reading occurs. An example of this proposal is the book trailer for the collection ¿Quién soy? by Seceda and García (2021)13 where narrators’ and characters’ voices combine in open spaces in which fiction intertwines with readers’ realities and the contents are revealed as being part of receivers’ own lives. In this sense, the promotional epitext of Brujas, guerreras y diosas by Hodges and Lee-Merrion (2020) is paradigmatic, as it is a document structured around testimonies from female readers who have chosen stories about fairies, vampires, sorceresses and goddesses with whom they identify and present them as contemporary role-models. Therefore, the reader’s reality and the fictional discourse are connected, thus removing the boundaries between reality and fiction. The book trailer for the picturebook ¿Debo argumentar el sinsentido de la esclavitud? by Frederick Douglass, with commentary by Squilloni and Fosch (2021) and translated by Jordi Pigem and Catarina Sacramento is of particular interest.14 It concerns the speech given by the ex-slave Frederick Douglass on Independence Day in the United States. The appeal to the reader, inherent in its ironic content, is made more powerful through the voices of the young black people who read aloud the first sentences of this rhetorical work of art while looking straight at the camera.

3.2.5. From the materiality of the discourse to in-depth reading

Another of the discourse markers present in promotional virtual epitexts in recent years is the relevance that the physicality of the act of reading acquires through the conception of the book as an object. While in previous studies (Tabernero-Sala, 2016b,2019) we insisted on the tendency to emphasize the materiality of the book as one of the categories used to define the book trailer, in the last three years this category has become so usual that it has been identified as a dominant tendency, which may be explained by the digital environment with which people engage. Thus, book trailers such as those relating to the promotion of Bienvenida by Comín (2021a),15 Mi arbolito de Navidad by Comín (2021b),16 Los volcanes by Geis (2022)17 or ¡ No chupes este libro! by Ben-Barak and Frost (2020)18 combine the dimension of the work as an object with specific references to its physical handling by the reader, at a leisurely pace that is a delight to the senses. This material dimension is part of the identification of in-depth reading to the development of the critical reader, involving the defense, as recent research recommends (Wolf, 2018; Zafra, 2019; Gil-Pelluch et al., 2020; Støle, 2020) of the time to think and memory retention that paper affords in contrast to the immediacy of the characteristic ‘skim reading’ of screens. The study conducted by Zafra (2017, p. 19), among others, refers to reading online every day as an experience based more ‘on impressions than on concentration,’ more on screenshots than on deliberate reflection. Zafra (2019) consequently defends the notion of ‘time to think’ as the only way to achieve autonomous, critical thinking.

3.2.6. The sensorial experience. The narrator’s voice

This register also includes the importance attributed to the narrator’s voice by the different promotional epitexts, whether it is the actual author, writer/illustrator, or oral narrators who read aloud in spaces physically suited to the content of the book. There is an increased presence of documents in which the narrator’s voice becomes one of the key elements of the promotional proposal. In this manner, book trailers such as the one for the picturebook ¡ Artista!, by Hernández-Sevillano and Cerro-Rico (2019),19 which presents a dramatized recitation of the text over the images, or the reading aloud of Nunca dejes de brillar by Alonso and Muñoz (2020)20 may serve as examples of pronounced discourse markers in the promotion of reading for children since the year 2020, closely linked to the necessity of defending reading as a sensorial experience.

3.2.7. The multimodal discourse

Cinematographic book trailers that develop a multimodal discourse in which music, image, animation and voice produce artistic documents with an entity of their own follow the same direction (Unsworth, 2015). Thereby, book trailers such as those for Bambi by Benjamin Lacombe (Salten and Lacombe, 2020),21 El bolero de Ravel by Abad and Delicado (2020),22 ShhTenemos un plan by Haughton (2019)23 or Abecedario by Kaufman and Franco (2017), Letra Ñ24 use ellipsis, metaphors and appeals to the reader in their composition to move readers and interact with them.

4. Discussion

In terms of specialized publishers’ promotion of children’s books, the analysis presented reveals that, in recent years, virtual epitexts have evolved in proportion to the new challenges posed by the digital society regarding reader training (Cordón-García, 2018, 2020; Lluch, 2018). The data examined from the sample selected reveal an increase in the number of virtual documents created by publishers relating to the dissemination of children’s books in recent years, with the peak incidence rate in 2020, possibly due to the changes produced by the COVID-19 health crisis. This fact is consistent with the results of the reports on post-pandemic reading habits among young people and adults (Cencerrado Malmierca and Yuste Tuero, 2020; ERI-Lectura, 2020; Sánchez-Muñoz, 2022). Similarly, the presence of the book trailer as a document to promote reading has decreased in comparison to less elaborate, more immediate virtual materials, as required by social media, where the ephemeral prevails over anything requiring a longer production time with the consequent economic costs involved. In response to the speed that characterizes the digital society, only a few publishers have opted to attend to the esthetic quality of virtual epitexts and offer what Unsworth (2015) terms ‘multimodal literary narratives’ in their digital extensions.

As far as the tendencies observed are concerned, the five macrocategories defined in the initial descriptive analysis identify significant differences compared to the virtual dissemination scenario of previous years (Lluch et al., 2015; Tabernero-Sala, 2016b). Materiality emerges as the tendency with the greatest presence in virtual promotional documents, in keeping with developments in the field of children’s book publishing (Kümmerling-Meibauer, 2015; Tabernero-Sala, 2016a,2019), although this is unevenly reflected in the documents analyzed, as some of them are simply recordings of a reader handling a book. Since 2020, the voices of authors and readers fill the virtual epitexts used in book promotion, emphasizing the documentary nature of the act of reading and reinforcing the presence of creators, mediators and readers in line with the overuse of the testimonial literary practices typical of the digital culture (Zafra, 2019).

To answer the question concerning the connection between promotional digital epitexts and the proposal of critical reading, we understand that what we have termed the ‘visible author’ (Sander’s, 2018) can be defined as one of the most significant discourse markers in the virtual epitexts selected in the qualitative content analysis. In this sense, the presence of the author in all aspects establishes cracks in the concept of ‘truth,’ by offering the reader a personal and ideological relationship to what is explained or related, and shows the mediator, and consequently the reader, the possibility of establishing methods of argumentation and questioning to use as a basis for the development of critical thinking, through the integration of different viewpoints, as advocated by Ennis (2011, 2018) and Kuhn (2018, 2019). It is not surprising that this concept of the ‘visible author’ includes the figure of the editor, thereby establishing the concept of a joint authorship that constantly addresses the reader. In the same vein, the empathic projection of the ‘visible author’ paves the way to raising receivers’ awareness, involving, once again, the connection between emotion and knowledge, one of the most significant aspects in contemporary theories on non-fiction picturebooks (Grilli, 2020; Tabernero-Sala, 2022). Likewise, promotional virtual epitexts should emphasize and encourage the presence of discourse markers linked to the hybridization of fictional and non-fictional discourses through the use of different types of language, leading to, as the case may be, what we have termed experiential reading. In this way, the promotion reinforces the relationship between the reader, the book and reality by suggesting the connection of the book with reading environments in which shared natural settings are the priority, in line with the proposals contained in the Sustainable Development Goals. The artistic and experiential component extends the relationship between the book and its environment, through feedback that transforms the individual’s relationship to their context, as studied in the ecocritical paradigm of research on children’s books (Goga et al., 2018).

The emphasis on the materiality of the book and the presence of voiceovers, oral narrators and musical discourses aimed to arouse emotion and wonder, as well as curiosity, recover the sensorial aspect of reading, since the act of reading engages the body (Littau, 2006). With materiality as a basis, in-depth reading is identifiable in the concept of multimodal reading, which requires the reader to pause (Wolf, 2018; Zafra, 2019; Gil-Pelluch et al., 2020; Støle, 2020) for a time, which results in deliberation and enjoyment typical of artistic discourse.

In summary, virtual epitexts for the promotion of children’s picturebooks may provide mediators and readers with means, such as those revealed in the selection above, with which to develop the basis of critical thinking and meet, in this way, the challenge posed by the digital society in training readers within the framework of a biliteracy that has already been established (Wolf, 2018). Following on from previous studies (Tabernero-Sala et al., 2022), the selected virtual epitexts present discourse markers typical of the promotion of the non-fiction picturebook, thereby confirming, as reported by recent studies (Von-Merveldt, 2018; Grilli, 2020; Salisbury, 2020; Dindelli, 2021; Jan, 2021), the vitality of this genre that has changed the panorama of children’s books by defining a new reading model based on the hybridization of fictional and non-fictional discourses according to the contexts of the reader and the mediator.

5. Conclusion

The analysis of the results defines this research based on the following findings:

• Firstly, new tendencies resulting from the challenges posed by the digital society in recent years and new literate practices are described from the perspective of digital promotion (Cordón-García and Muñoz-Rico, 2023).

• Secondly, the results distinguish discourse markers that favor critical reading, which was previously non-existent in promotional virtual epitexts. The visibility of the author, the removal of the boundaries between reality and fiction, the experiential connection between the author, the reader and their environment, and the enhancement of the material aspects of the discourse all emphasize in-depth reading through the use of digital promotion (Wolf, 2018), along the lines established in the premises on critical reading advocated in international documents, such as Reimagining our Futures Together: A New Social Contract for Education (Comisión Internacional sobre los Futuros de la Educación, 2022).

• It is therefore understood that promotional virtual epitexts create meaning (Gray, 2010) and propose, from the digital paradigm, a way of reading that fosters the essential analogical aspect of in-depth reading, which is key to critical thinking. Consequently, a corpus such as the one selected for this research could be defined as a selection of recommended good practices in the context of training future mediators (Trigo-Ibáñez and Santos-Díaz, 2023; Álvarez-Ramos et al., 2023).

This opens future lines of research to delve into the analysis of the virtual epitexts used in the promotion of reading and into the reading paradigm that they present. Similarly, from this perspective, there is a need for research into how social media influence the contents and methods used in the promotion of reading in the digital society. Finally, the study of model virtual epitexts should continue to transfer the results to reading mediators.

6. Limitations

This research was essentially conducted in the context of Spanish publishing, although some Latin American publications were also selected, since the main criterion was for the works to be published in Spanish. Therefore, we consider that the sample of 836 audio-visual documents is representative for the research objectives but the results are not generalizable to other contexts. Furthermore, the audio-visual documents in the sample have been taken from two digital media, YouTube and Vimeo, given that other social media replicated content and were more unstable; this means that some publishers who had abandoned these platforms may have produced significant publications via other digital media on the dates indicated that have not been registered. Regarding the method used, the approach is quantitative as regards data registration and detection of the initial categories, by frequency and saturation, and qualitative-interpretive as regards content analysis and reporting of results. As a consequence, the limitations involved in research with these characteristics, concerning representativeness or generalization, should be assumed in the interests of the reflection and in-depth analysis required by the objectives of this study as a contribution to educational research.

Data availability statement

The original contributions presented in this study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.

Author contributions

RT-S: conceptualization, analysis, writing of the manuscript, review, and supervision. MC-C: methodology, analysis, editing, review, and supervision. Both authors contributed to the article and approved the submitted version.

This research was framed within the R&D&I Project entitled “Non-fictional readings for the integration of critical citizens in the new cultural ecosystem. RENFICE” (PID2021-126392OB-I00), financed by the Spanish Ministry of Science and Innovation.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Grilli, G. (2020). “Beauty and the World. Some questions to five illustrators (often also authors) of non-fiction picturebooks for children on their work, poetics, inspiration(s),” in Non-fiction picturebooks. Sharing knowledge as an esthetic experience, ed. G. Grilli (Pisa: Edizioni ETS), 267–293.

Small Space Vegetable Gardening Topic of Evening Garden Affair

Hardin County – The Hardin County OSU Extension Master Gardener Volunteers are hosting “An Evening Garden Affair” on Monday evening, June 26 at the Friendship Gardens of Hardin County located at 960 W. Kohler Street in Kenton.

The event is from 6:30 to 8:00 pm and will feature John King, Hancock County Master Gardener Volunteer, and part-time horticulturist with the Findlay Country Club. He is a 1983 graduate of the University of Kansas where he received a degree in Biology.

The topic of the program will be “Small Space Vegetable Gardening.” King will discuss the advantages and disadvantages of growing vegetables in pots and grow bags as well as the square foot gardening method, touching on the Mittleider gardening method. The Mittleider gardening method was developed for growing plants in a soilless medium to create high yield vegetable gardens under precisely controlled feeding and watering conditions.

Attendees will gather in the Friendship Gardens of Hardin County for tours at 6:30 pm with Hardin County OSU Extension Master Gardener Volunteers. Learn about the many different themed gardens at this educational demonstration garden. There will be snacks and drinks available, along with free plants to lucky attendees before moving to Simon Kenton School gymnasium with seating at 7:00 pm for the program presented by speaker John King.

This event is free and open to the public, rain, or shine. Those who have an interest in gardening will not want to miss this event. Parking is available at the garden off West Kohler Street or in front of Simon Kenton School.

For further information contact the OSU Extension office at 419-674-2297.

This content was originally published here.

Camps Focus on Fun This Summer

The fun, sun-filled days of summer are right around the corner, and the St. Charles Park District has just the thing to keep kids active and engaged all season long.

Summer camps, all led by experienced counselors who have been trained in CPR and first aid, will meet weekly from May 30 to August 11. Registration is open now

“We offer a lot of variety and there are many on-site activities we can provide,” said Jaz Pacheco, who heads several of the park district’s camps.

Flex Camp
Schedule your child’s day of fun around your family summer schedule by registering for 2, 3, 4 or 5-day camp each week.

Kids can make friends while playing outdoor group games and taking part in arts and crafts. Various camps will offer trips to the playground, minigolf, Otter Cove Aquatic Park and Swanson Pool. Flex Camp is available to those entering kindergarten (every child must be 5 by September 1) up to those entering fifth grade.

In addition to Flex Camp, the St. Charles Park District also offers 5-day-a-week Summer Camp for those entering first through fourth grades. Both camps meet at Pottawatomie Park.

Little Scouts
Those too young to join Flex or Summer Camp are invited to take part in Little Scouts. Geared to kids ages 3 to 5, this program takes place for two weeks on Tuesdays and Thursdays, with three-hour morning and afternoon sessions available beginning June 6 through August 3. Led by staff of the Pottawatomie Preschool, campers will spend time playing with friends on the playground and participating in group activities.

Teen Camp
Teen Camp is aimed at those entering sixth through eighth grade, and includes local and area day trips as well as sports, minigolf and trips to the pool and Primrose Farm. Field trips outside of the park district grounds include Raging Waves Waterpark in Yorkville, Santa’s Village Amusement Park in East Dundee and the Museum of Science and Industry downtown Chicago, among other exciting destinations. Camp meets at Baker Community Center.

Nature Camps
Various nature camps will help young children as young as 2 up to those entering fifth grade develop a deep appreciation for the outdoor world.

Wee Wonders Camp leads kids 2 to 4 on a path of discovery as, together with a parent, they learn about local wildlife and insects. The camp offers three different programs. Hopping through Habitats will take place June 20 and 22; Eric Carle & Friends on July 18 and 20; and Insect Investigators on August 1 and 3. This camp takes place for an hour and a half and is suitable for children who have not attended preschool.

Nature Discoverers Camp is a great option for budding naturalists, ages 3 to 5, who wish to explore the woods, fields and other natural spaces. Weekly camps take place for two hours Monday through Friday, June 5 through August 4. Campers should have attended preschool and be potty trained.

Pathfinder Camp for those entering first and second grade and Trailblazer Camp for children entering third through fifth grade will foster an explorer’s spirit in campers as they hike, meet and greet local fauna and take part in nature-based crafts. The camps, which will take place for six hours Monday through Friday, will introduce kids to a new theme each week.

Learn more about summer camp offerings at stcparks.org/camps-faqs. To register, visit www.stcparks.org/camps.

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Tuesday Tip: Quick Hack to Help, Bitte

Neko Case – “I’m an Animal

New Mexico – White Sands National Park.

A number of people have told me they don’t want to say anything publicly about Losing My Religions because they don’t want to be associated with some of the opinions expressed, or they work with some of the organizations. I understand, which is why the reviews on the book site are anonymous.

However, you can give the book a rating without subjecting yourself to the wrath of angry vegans or climate catastrophists! And ratings and reviews really help.

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* Latest review at Amazon:

I received an ARC from Matt and love what he’s written. His weaves a non-stop stream of energy, nostalgia, and purpose into a real yet funny read. Passages like the following are the norm, rather than exception, and keep me laughing and on my toes:

It might sound like I was rebellious, but in reality, I was an obedient Catholic boy, serving mass when others wouldn’t – even while sick, barfing in the vestibule. Like PETA co-founder Alex Pacheco a few years before me, I thought I might want to be a priest. (The local girls seemed to think I should be celibate.) Luckily, Father Ted looked me straight in the eyes and said, “No. No, you don’t.”

Frontiers | Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure

1. Introduction

Heart failure (HF) can arise from structural or functional abnormalities of the heart due to various reasons and is the final stage of multiple cardiovascular diseases (Mcmurray et al., 2012).

With improvements in global economic levels, changes in human dietary structures, and poor lifestyle habits, HF has become a worldwide public health issue. A systematic review by Askoxylakis et al. revealed that the 5-year mortality rate of chronic heart failure is essentially equal to that of cancer (Askoxylakis et al., 2010). Studies indicate that there are approximately 26 million heart failure patients globally, with a prevalence of 1.5% to 2.0% in developed countries, and over 10% among individuals aged 70 and above (Mosterd and Hoes, 2007).

With the development of modern medicine, researchers have deepened their understanding of HF, leading to changes in concepts, innovations in methods, and updates in guidelines. While the in-hospital fatality rate of HF patients has shown a clear downward trend, it is worth noting that the re-hospitalization rate continues to increase (Hu, 2021). Therefore, it is still a hot and difficult topic in clinical research to improve the quality of life, reduce the fatality and re-hospitalization rate, and thus promote the long-term prognosis (Mao et al., 2021).

The intestine, also known as the second brain, is the body’s largest digestive and excretory organ (Mayer, 2011). The intestinal microbiota primarily consists of Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Verrucomicrobia (Eckburg et al., 2005), with over 90% of bacteria in a healthy gut classified as Bacteroidetes and Firmicutes (Gill et al., 2006). Under normal physiological conditions, the host provides a suitable environment and necessary nutrition for gut microbiota, which in turn participate in various biological functions, such as nutrient metabolism and absorption, energy balance, neural development, immune regulation, and maintenance of intestinal mucosal barrier defense (Everard and Cani, 2014). This creates a balanced, symbiotic, and ecological environment, leading to the gut microbiota gene being referred to as the second genome (Mayer, 2011). In an unbalanced state, gut microbiota dysbiosis can impact the host’s growth, development, health and disease, and drug treatment (Hooper and Gordon, 2015). The disorder is closely related to the onset and progression of various diseases, including those in the digestive system (Larsson et al., 2012), mental system (Wang B. et al., 2017), endocrine system (Bäckhed et al., 2004; Qin et al., 2012; Tremaroli and Bäckhed, 2012), and autoimmune system (Tremaroli and Bäckhed, 2012), as well as some infectious diseases (Wang et al., 2014). Gut microbiota dysbiosis and its metabolites play a vital role in the occurrence and development of HF (Sandek et al., 2007a; Andreas et al., 2014). Therefore, interventions targeting gut microbiota dysbiosis, improving intestinal membrane barrier function and intestinal wall permeability, and reducing endotoxin absorption and inflammation may alleviate myocardial damage, suggesting a new direction for HF treatment in the future.

Qishen granule (QSG), a clinically approved traditional Chinese medicine, has been researched for treating HF for many years (Wang J. et al., 2017). QSG is composed of six botanical drugs, including Astragalus camptoceras Bunge (Fabaceae), Aconitum carmichaelii Debeaux (Ranunculaceae), Salvia miltiorrhiza Bunge (Lamiaceae), Scrophularia ningpoensis Hemsl. (Scrophulariaceae), Lonicera japonica Thunb. (Caprifoliaceae), and Glycyrrhiza uralensis Fisch. ex DC. (Fabaceae) (30: 9: 15: 10: 10: 6) (Chen et al., 2022; Li et al., 2022). Our previous study detailed its preparation process and composition identification (Wang et al., 2012; Xia et al., 2017).

However, the effect of QSG on intestinal microecology remains unconfirmed. Therefore, this study aimed to elucidate the potential mechanism of QSG regulating HF in rats, based on intestinal microecological changes.

2. Materials and methods

2.1. Experimental animals and Ethics Statement

Male Sprague–Dawley rats (180 ± 10 g) were provided by the Vital River Laboratory Animal Technology Co. Ltd. (Beijing, China). The animal housing conditions were maintained at 23 ± 2°C, 40 ± 5% relative humidity, and 12:12 h light–dark cycles. Rats were adaptively fed for 1 week. All experimental procedures were conducted and supervised by the Animal Care Committee of Beijing University of Chinese Medicine, in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals.

2.2. Drugs

Qishen Granule is composed of six botanical drugs, including Astragalus camptoceras Bunge (Fabaceae), Aconitum carmichaelii Debeaux (Ranunculaceae), Salvia miltiorrhiza Bunge (Lamiaceae), Scrophularia ningpoensis Hemsl. (Scrophulariaceae), Lonicera japonica Thunb. (Caprifoliaceae), and Glycyrrhiza uralensis Fisch. ex DC. (Fabaceae) (30: 9: 15: 10: 10: 6) (Chen et al., 2022; Li et al., 2022), and its composition was identified by high-performance liquid chromatography (Wang et al., 2012; Xia et al., 2017).

Trimetazidine, used as the positive drug, was purchased from Servier (Tianjin) Pharmaceutical (National drug approval number H20055465).

2.3. HF model induction and Electrocardiogram

As previously described (Gao et al., 2020), ligation surgery of the left anterior descending (LAD) coronary artery was performed on anesthetized rats using intraperitoneal injection of 1% pentobarbital sodium (45 mg/kg). Briefly, a left thoracotomy was performed between the third and fourth intercostal spaces in the rats. After exposing the cardiac tissues, the LAD was ligated with a sterile suture (Shuangjian, Shanghai, P. R. China) 1 mm below the left atrium. The thorax was then closed layer by layer. After thoracotomy, rats were warmed on a heated blanket. Sham-operated rats underwent the same procedure without LAD ligation. On the third day after surgery, the rats were anesthetized by intraperitoneal injection of 1% pentobarbital sodium at 40 mg/kg, and the presence of 6–8 pathological Q-waves in the electrocardiogram indicated successful surgical ligation (result shown in Supplementary Figure 1).

2.4. Animal grouping and drug administration

Rats with successful HF models were randomly divided into the Model group (Model), Qishen granule group (QSG), and trimetazidine group (TMZ), with 12 animals in each group. As previously described (Gao et al., 2020), rats were treated with intragastric administration at a daily dose of 18.66 g/kg for 28 days. Rats in the TMZ group received 6.3 mg/kg of TMZ. Rats in the sham operation group and model group were administered an equal volume of normal saline intragastrically for 28 days.

2.5. Assessment of cardiac functions by echocardiography

As previously described (Gao et al., 2020), M-mode echocardiography was used to measure the internal diameter of the left ventricle at the end of systolic/diastolic periods and the thickness of the anterior/posterior left ventricle. Then left ventricular ejection fraction (LVEF), Z left ventricular fractional shortening (LVFS), left ventricular anterior wall; diastolic (LVAW;d), left ventricular anterior wall; systolic (LVAW;s), left ventricular internal end-diastolic diameter (LVID;d), left ventricular internal end-systolic diameter (LVID;s), left ventricular posterior wall; diastolic (LVPW;d), and left ventricular posterior wall; systolic (LVPW;s) were calculated to evaluate the cardiac systolic function and myocardial hypertrophy.

2.6. Hematoxylin–eosin (HE) and Masson staining

The heart and ileum tissues were fixed in 4% paraformaldehyde for more than 48 h, embedded in paraffin, and sectioned at 5 μm thickness for further histological analysis. Hematoxylin–eosin (HE) staining was performed for the heart and ileum tissues, and Masson staining was performed for the heart tissues to visualize tissue architecture.

2.7. Mitochondrial ultrastructure observation using transmission electron microscopy

Cardiac tissues from the infarct border zone of the left ventricle (1 mm × 1 mm × 2 mm) were fixed in 4% glutaraldehyde (2 h), in 1% osmic acid (2 h), and then washed with phosphate-buffered saline (PBS) solution three times (5 min). Ultrastructural alterations were observed using a transmission electron microscope (Hitachi, Tokyo, Japan) after dehydration, permeation, embedding, and ultrathin sections were cut.

2.8. LPS detection

Lipopolysacchride detection was measured following the instructions of the test kit (20152400090, Fuzhou Xinbei Biochemistry Industry Co., Ltd).

2.9. Detection of gut microbiota

Fresh fecal samples (1 g) were collected from rats and placed in a 30 ml sterile tube containing 15 ml of phosphate-buffered saline (pH 7.2). The samples were mixed and centrifuged at 200 RPM for 10 min. After removing the sediment, 200 ul of suspension was obtained after oscillation. DNA extraction, PCR amplification, Illumina MiSeq sequencing, and processing of sequencing data are described in detail in the Supplementary Material. The alpha diversity indexes, including species rarefaction curve, richness index (Sobs index), and diversity index (Shannon index), were analyzed using Mothur (Schloss et al., 2011) (version v.1.30.1), and the similarity level of operational taxonomic units (OTUs) for index evaluation was 97% (0.97). Beta diversity analysis was performed to compare differences among different groups, including partial least squares discriminant analysis (PLS-DA) and linear discriminant analysis effect size (LEfSe).

The raw reads were deposited into the NCBI Sequence Read Archive (SRA) database (Accession Number: SRP431350).

2.10. Statistical analysis

Data are presented as the mean ± standard error (X ± SEM). One-way ANOVA or Kruskal–Wallis H analysis of variance were used to detect statistically significant differences (P < 0.05) among groups. A community histogram was drawn using GraphPad Prism (version 9.0), and Venn diagrams, heatmaps, and PLS-DA plots were created using R packages.

3. Experimental results

3.1. QSG improved cardiac functions in HF rats

Echocardiography results (Figure 1) demonstrated significant downregulation of LVEF, LVFS, LVAW;d, LVAW;s, LVPW;d, and LVPW;s (P < 0.001, P < 0.01) and upregulation of LVID;d and LVID;s (P < 0.001, P < 0.01) in the model group compared to the sham group. QSG and TMZ significantly increased LVEF, LVFS, and LVAW;d (P < 0.01, P < 0.05), while QSG also significantly improved LVID;s (P < 0.05).

FIGURE 1

Figure 1. QSG improved cardiac function and reduced pathological changes in HF rats. (A) Sham group. (B) Model group. (C) Qishen granule group. (D) TMZ group. (E–L) LVEF, LVFS, LVAW;d, LVAW;s, LVID;d, LVID;s, LVPW;d, and LVPW;s N = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. model group.

3.2. QSG reduced pathological changes in HF rats

Based on HE staining (Figures 2A–D), cardiomyocytes in the sham group were tightly arranged and orderly, while those in the model group were loosely arranged with obvious inflammatory cell infiltration and pyknotic dark-staining nuclei. Compared with the model group, QSG and TMZ attenuated these pathological changes.

FIGURE 2

Figure 2. HE (A–D) and Masson (E–H) staining. (A/E) Sham group. (B/F) Model group. (C/G) Qishen granule group. (D/H) Trimetazidine group. Blue arrowheads indicate the intercellular space and green arrowheads indicate inflammatory infiltration (A–D). Blue-stained fiber indicates collagen deposition (E–H). Scale bar = 100 μm.

Masson staining (Figures 2E–H) showed that, compared to the sham group, the cardiomyocytes in the model group were necrotic and replaced by extensive collagen fibrous tissues with diffuse and infiltrating distributions. Both QSG and TMZ significantly inhibited collagen deposition.

3.3. QSG improved the structure of mitochondria

Transmission electron microscopy results (Figure 3) revealed that in the sham group, mitochondria with a complete membrane structure were round or oval and densely and orderly arranged. The mitochondrial cristae and matrix were arranged evenly and clearly (Figure 3A). In contrast, the model group exhibited scattered arrangement, obvious swelling, a loose matrix, and evident partial fracture cristae (Figure 3B). QSG and TMZ significantly improved the structure of mitochondria.

FIGURE 3

Figure 3. Transmission electron microscopy of myocardial tissue. (A) Sham group. (B) Model group. (C) Qishen granule group. (D) Trimetazidine group.

3.4. QSG reduced pathological changes in the ileum

Compared to the sham group, the model group exhibited significantly lower epithelium and ileum villi heights. The ileum villi in the sham group were neatly ordered and tightly arranged, whereas in the model group they were rough, swollen, and irregularly arranged. QSG and TMZ improved the above pathological changes (Figure 4).

FIGURE 4

Figure 4. HE staining of ileum tissue. (A) Sham group. (B) Model group. (C) Qishen granule group. (D) Trimetazidine group. Blue arrowheads indicate the epithelium and green arrowheads indicate the ileum villi. Scale bar = 200 μm.

3.5. OTUs, Venn, and alpha microbial analysis

A total of 2,732,663 raw reads were obtained from intestine microbiota by 16S rRNA Illumina sequencing, with an average of 53,457 clean reads per sample after quality control and read assembly. The amount of sequencing data was sufficient.

In total, 999 OTUs were identified, with the Venn diagram showing that 578 (57.86%) ere shared among the four groups and 118 (11.8%) OTUs were unique to each group (Figure 5A). On the genus level, after matching and identification, 999 OTUs were mapped to a total of 213 genera, with 139 (65.26%) shared among the four groups and 21 (9.86%) unique to each group (Figure 5B).

FIGURE 5

Figure 5. OTUs, Venn, and alpha microbial analysis. (A) Venn result of microbial analysis on the level of OTUs. (B) Venn result of microbial analysis on the level of genus. (C) Community richness. (D) Community diversity. N = 10 per group; QSG, Qishen granule; TMZ, trimetazidine.

Although there was no significant difference in community diversity (Shannon) among the four groups (Figure 5D), community richness (Sobs) was increased in the model, QSG, and TMZ groups compared to the sham group (Figure 5C). The rarefaction curve, constructed by community richness (Sobs), is shown in Supplementary Figure 3.

3.6. PLS-DA and percent of community abundance among the four groups

PLS-DA analysis of the phylum level showed that the sham, QSG, and TMZ groups were clustered together, separated from the model group (Figure 6A), and that the main phyla were Firmicutes and Bacteroidetes (Figure 6C). PLS-DA analysis of the genus level showed that samples from the four groups were separated from others (Figure 6B), indicating that QSG and TMZ influenced the community composition. The main genus is shown in Figure 6D.

FIGURE 6

Figure 6. PLS-DA and percent of community abundance among the four groups. (A) PLS-DA analysis of the phylum level. (B) PLS-DA analysis of the genus level. (C) Percent of community abundance on the phylum level. (D) Percent of community abundance on the genus level.

3.7. LEfSe and phenotype prediction among the four groups

LEfSe analysis was used to screen microbes differentially among species. The bar chart indicated that 25 specific taxa were identified (threshold value of LDA = 3), with 1 in the sham group, 4 in the model group, 8 in the QSG group, and 12 in the TMZ group (Figure 7A). Furthermore, according to BugBase phenotype prediction, there was no significant difference in the composition of gram-negative or gram-positive bacteria among the four groups (Figures 7B, D). The determination of plasma LPS showed that QSG and TMZ significantly reduced LPS caused by HF (Figure 7C).

FIGURE 7

Figure 7. LEfSe and phenotype prediction among the four groups. (A) LEfSe analysis. (C) LPS detection. (B, D) Phenotype prediction. *P < 0.05, **P < 0.01; QSG, Qishen granule; TMZ, trimetazidine.

4. Discussion

Previous studies have shown that QSG is effective in treating HF (Wang B. et al., 2017; Chen et al., 2022; Li et al., 2022). However, the effect of QSG on intestinal microecology has not been confirmed. Therefore, this study aimed to elucidate the possible mechanism of QSG regulating HF in rats based on intestinal microecological changes. The findings can be summarized as follows: (1) QSG administration improved cardiac function, tightened cardiomyocytes alignment, decreased fibrous tissue and collagen deposition, and reduced inflammatory cell infiltration, basically consistent with previous study conclusions (Li et al., 2016). (2) The results of electron microscopic observation of mitochondria showed that QSG could arrange mitochondria neatly, reduce swelling, and improve the structural integrity of the crest. (3) Firmicutes were the dominant component in the model group, and QSG could significantly increase the abundance of Bacteroidetes and Prevotellaceae_NK3B31_group. (4) Furthermore, QSG significantly reduced plasma LPS, improved intestinal structure, and recovered barrier protection function in rats with HF.

The gastrointestinal barrier consists of mechanical, immune, chemical, and biological barriers. In a normal state, harmful intestinal substances, such as bacteria and their related endotoxins, are prevented from entering other host body tissues and the bloodstream through the intestinal mucosa (Sandek et al., 2007b; Lozupone et al., 2012). In recent years, an increasing number of studies have supported the role of the gut in the pathogenesis of HF, known as the “gut hypothesis of HF.” The hypothesis suggests that reduced cardiac output and increased systemic congestion may lead to ischemia and/or edema of the intestinal muscles with HF (Sandek et al., 2007a), characterized by hypoperfusion, ischemia, hypoxia of the intestinal mucosal, congestion, increased permeability, and reduced absorption of nutrients such as sugars, proteins, and fats. These factors can change the abundance and composition of gut microbiota, leading to increased bacterial translocation and circulating endotoxin, such as LPS (Andreas et al., 2014). Translocated microbiota and increased LPS exacerbate intestinal barrier function damage, stimulate an inflammatory response, and accelerate the pathological development of HF (Organ et al., 2016).

Bacteroidetes are absolutely dominant in gut microbiota, participating in the metabolism of various substances, fermenting carbohydrates, polysaccharides, steroids and bile acids, promoting the formation of intestinal mucosa vessels, preventing intestinal inflammation (Brown et al., 2019), maintaining intestinal physiological functions, and exerting a significant influence on hosts’ health (Yu et al., 2015). Compared to healthy individuals, the abundance of Bacteroidetes (the genera Bacteroides and Prevotella) in patients with coronary heart disease was significantly decreased, and the ratio of Firmicutes/Bacteroidetes was increased (Emoto et al., 2017), which was associated with many potential cardiovascular diseases. Jie conducted a whole-genome study on fecal samples of 218 patients with coronary heart disease and 187 healthy individuals, showing that the abundance of Bacteroides and Prevotella was relatively reduced in the former group (Jie et al., 2017). Tan sequenced the gut microbiota of 36 patients with ischemic cardiomyopathy with different cardiac function levels and found that the proportion of Firmicutes was closely related to the occurrence of ischemic cardiomyopathy, with HF severity increasing alongside Firmicutes abundance (Tan, 2018). In Li’s study on individuals over 60 years old, LEfSe analysis showed that Bacteroidetes were more abundant in the healthy group, while Firmicutes and Enterobacterium were more abundant in the HF group (Li, 2019). Romano identified eight intestinal bacteria belonging to Firmicutes, which significantly decomposed choline and produced trimethylamine (Romano et al., 2015), and then trimethylamine oxide (TMAO) promoted myocardial microangiopathy in non-ischemic HF patients. Elevated TMAO predicted adverse events in both non-ischemic and ischemic HF patients (Rhee et al., 2013). Yu found that the average abundance of Bacteroidetes was 66.23 ± 5.11% in the clinically healthy control group, 45.69 ± 4.63% in the coronary heart disease group, and 27.89 ± 2.39% in the coronary heart disease combined with the HF group (Yu et al., 2021).

Hypertension (Marques et al., 2017), hyperlipidemia (Suparna, 2015), and obesity (Rastmanesh, 2011) are common risk factors for HF, and the ratio of Firmicutes/Bacteroidetes increases with the worsening of adverse degree.

In this study, Firmicutes abundance was the dominant component in the model group, and QSG significantly increased the abundance of Bacteroidetes, which might be one of the targets of QSG for cardiac protection.

Purushe et al. stated that Prevotella was related to the biosynthesis of short-chain fatty acids (SCFAs), which could supply nutrients for intestinal epithelial cells and maintain the intestinal mucosal barrier and an acidic pH environment to prevent the invasion of related pathogenic microorganisms. A lack of SCFAs might reduce the protective effect of the intestinal mucosal barrier and lead to increased levels of enterotoxin (Purushe et al., 2010; Shen et al., 2017). Tang et al. (2018) found that dietary supplements provided with SCFAs within 24 h after myocardial infarction significantly reversed the high mortality and ventricular rupture rates caused by broad-spectrum antibiotics. Kovatcheva et al. found that sugar metabolism could be improved by supplementing with prebiotics containing Prevotella (Petia et al., 2015). In a study of patients with chronic renal failure, Xie et al. found that the abundance of Prevotellaceae belonging to Bacteroidetes decreased significantly (Xie, 2014). In the mouse model of ulcerative colitis induced by glucan sodium sulfate, disturbed gut microbiota might aggravate intestinal mucosal barrier damage by reducing the thickness of the intestinal mucus layer. Huangqin Decoction could significantly improve the abundance of Prevotellaceae and maintain the function of the intestinal mucosal barrier (Xu, 2018). According to Liu et al. (2018) and Smith et al. (2019), the abundance of Muribaculaceae and Prevotellaceae_NK3B31_group was closely related to the generation of SCFAs. Additionally, the reduced abundance of the Prevotellaceae_NK3B31_group was associated with inflammation (Wu, 2018). A study on Lingguizhugan Decoction showed that the herbs group could increase the abundance and diversity of gut microbiota in mice with HF, regulate the disorder, and increase the abundance of gut microbiota associated with SCFAs production, such as norank_f_Muribaculaceae and Prevotellaceae_NK3B31_group (Zhang et al., 2023). In this research, QSG significantly increased the abundance of Prevotellaceae_NK3B31_group.

Treg cells transcribe Forkhead box protein3 (Foxp3) and maintained human immune tolerance (Figueiredo and Schumacher, 2016), and the expression level was positively correlated with the relative abundance of Izimaplasmatales (Zhang, 2021). Izimaplasmatales were significantly underrepresented in the diabetes model (Niu et al., 2022) and obesity group (Zhang, 2021). Turicibacter was associated with the production of butyric acid (Zhong et al., 2015), and the colonization of Turicibacter sanguinis could reduce the overall triglyceride level and groin fat cell size of the host (Wu et al., 2020). In this study, the abundance of Izimaplasmatales and Turicibacter was the dominant composition in the TMZ group.

Pasini et al. (2016) compared 60 HF patients with healthy individuals and found that intestinal permeability in patients increased by 78.3%, and the number of patients with cardiac function grades III and IV (NYHA grade) was higher than those with grades I and II. Sandek’s team showed that, compared to the healthy control group, intestinal arterial blood flow in patients with HF was reduced by 30% to 43%, and the decreased intestinal arterial blood flow was correlated with the severity of HF (Sandek et al., 2014). Intestinal transport function was reduced by 54% in patients with HF, and it was more notable in HF patients with edema (Sandek et al., 2012). These data imply that the assessment of intestinal barrier function may contribute to the understanding of the gut-directed treatment of HF. In this research, QSG could improve the intestinal morphology of rats with HF.

Gut microbiota participates in food digestion through two major metabolic pathways, including sugar and protein decomposition (Sekirov et al., 2010). Meanwhile, gut microbiota can affect the host in various ways. To associate with other organs, the gut microbiota needs to release signaling molecules, which in some cases are microbial physical compositions, such as LPS. LPS typically interacts with host cells’ surfaces through pattern recognition receptors (PRRs) (Larsson et al., 2012). PRRs recognize pathogen-related molecular and can stimulate immune responses (Brown and Hazen, 2014).

The relationship between systemic inflammation and associated bacterial migration in HF has been observed and documented. Specifically, elevated levels of endotoxins, particularly LPS, in HF patients initiated signaling cascades that increased the production of cytokines, such as tumor necrosis factor-α (TNF-α) and aggravated HF (Niebauer et al., 1999; Sandek et al., 2007a). Moreover, LPS levels in the hepatic veins of HF patients were significantly higher than those in other circulatory sites, including the left ventricle and pulmonary artery, suggesting that HF exacerbation might result from excessive endotoxin influx from the gut into the bloodstream (Peschel et al., 2003), preliminarily confirming the link between gut microbiota and HF.

LPS induces pro-inflammatory damage by binding its lipid moiety, lipid A, to Toll-like receptor 4 (TLR4) (Poltorak et al., 1998). This binding leads to the recruitment of the adaptor protein myeloid differentiation primary response protein 88 (MyD88) to the cytoplasmic domain of TLR4, resulting in the activation of the transcription factor NF-κB (Liu et al., 2017) and the expression of NLRP3, GSDMD, and IL-1 in downstream inflammatory pathways, ultimately promoting HF development (Violi et al., 2023). Interestingly, lipid A exhibits structural variation. Lipid A in Bacteroides LPS is penta- or tetra-acylated, which reduces TLR4 responses (Vatanen et al., 2016; d’Hennezel et al., 2017; Wexler and Goodman, 2017).

In this research, QSG improved intestinal morphology and reduced serum LPS content in HF rats. In previous QSG studies (Chen et al., 2022; Li et al., 2022), GSG protected the heart by inhibiting the TLR4/NF-κB pathway in both rat and mouse models.

Astragalus camptoceras Bunge (Fabaceae) was one of the main herbs in QSG, and other drugs in it demonstrated therapeutic effects on the intestine and gut flora. On the basis of conventional anti-inflammatory drugs, Astragalus granules combined with Bifidobacterium quadruple viable tablets effectively treated ulcerative colitis patients, improving T lymphocyte subset levels, reducing inflammation, and inhibiting disease activity, with good safety (Zhu, 2022). Astragalus polysaccharide increased the abundance of Bifidobacteria and lactobacilli, while decreasing enterobacteria and enterococci in the stool of rats with ulcerative colitis, thereby improving intestinal flora imbalance (Liang et al., 2012).

5. Conclusion

The holistic view of traditional Chinese medicine posits that the host and its environment are inseparable, and although gut microbiota reside within the host’s body, they also belong to the “external environmental conditions” (Gao, 2004). A dynamic balance between the host and gut microbiota is required for maintaining normal physiological functions.

HF pathogenesis is complex, involving various physiological reactions, metabolic pathways, and signaling pathways, and results from comprehensive functional disorders. Gut microbiota dysbiosis and its metabolites play crucial roles in the occurrence and development of HF. Interventions targeting gut microbiota dysbiosis, improving intestinal barrier function and permeability, and reducing endotoxin absorption and inflammation may alleviate myocardial damage, suggesting a novel approach to HF treatment. The data presented here demonstrate that QSG improves cardiac function by regulating intestinal microecology in HF rats, highlighting promising therapeutic targets for HF.

However, it is important to recognize that the disease mechanisms caused by gut microbiota dysbiosis have not been fully elucidated and targeted interventions for gut microbiota in treating cardiovascular diseases, such as HF, have not been widely implemented in clinical practice. Therefore, the causal relationship and more detailed mechanisms between gut microbiota, their metabolites, and HF require further investigation.

Data availability statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary material.

Ethics statement

The animal study was reviewed and approved by Beijing University of Chinese Medicine.

Author contributions

KG, RY, and CW conceived this research. KG, XY, FL, YH, JL, and SL contributed to the process of experiment, sample collection, and data analysis. The first version of the article was written by KG and revised by XY. FL and LL performed the final review and contributed to the project administration. All authors contributed to the article and approved the submitted version.

This research was supported by the National Natural Science Foundation of China (81973680 and 81903950) and Scientific Launching Research Foundation of Beijing University of Chinese Medicine (2022-JYB-XJSJJ008).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

DNA, Deoxyribonucleic acid; LVEF, Left ventricular ejection fraction; LVFS, Left ventricular fractional shortening; HE, Hematoxylin; HF, Heart failure; LAD, Left anterior descending; LEfSe, Linear discriminant analysis effect size; LPS, Lipopolysaccharide; LVAW;d, Left ventricular anterior wall; diastolic; LVAW;s, Left ventricular anterior wall; systolic; LVID;d, Left ventricular internal end-diastolic diameter; LVID;s, Left ventricular internal end-systolic diamete;r LVPW;d, Left ventricular posterior wall; diastolic; LVPW;s, Left ventricular posterior wall; systolic; OUT, Operational taxonomic units; PBS, Phosphate-buffered saline; PLS-DA, Partial least square-discriminant analysis; PRRs, Pattern recognition receptors; QSG, Qishen granule; rRNA, Ribosomal RNA; SCFAs, Short-chain fatty acids; SEM, Standard error; SRA, Sequence read archive; TLR4, Toll-like receptor 4; TMAO, Trimethylamine N-oxide; TMZ, Trimetazidine; TNF-α, Tumor necrosis factor-A.

Brown, E. M., Ke, X., Hitchcock, D., Jeanfavre, S., Avila-Pacheco, J., Nakata, T., et al. (2019). (2019). Bacteroides-derived sphingolipids are critical for maintaining intestinal homeostasis and symbiosis. Cell Host Microbe 25, 668–680. doi: 10.1016/j.chom.2019.04.002

Chen, X., Li, Y., Li, J., Liu, T., Jiang, Q., Hong, Y., et al. (2022). Qishen granule (QSG) exerts cardioprotective effects by inhibiting NLRP3 inflammasome and pyroptosis in myocardial infarction rats. J. Ethnopharmacol. 285, 114841. doi: 10.1016/j.jep.2021.114841

Emoto, T., Yamashita, T., Kobayashi, T., Sasaki, N., Hirota, Y., Hayashi, T., et al. (2017). Characterization of gut microbiota profiles in coronary artery disease patients using data mining analysis of terminal restriction fragment length polymorphism: gut microbiota could be a diagnostic marker of coronary artery disease. Heart Vessels 32, 39–46. doi: 10.1007/s00380-016-0841-y

Marques, F. Z., Nelson, E., Chu, P. Y., Horlock, D., Fiedler, A., Ziemann, M., et al. (2017). High-fiber diet and acetate supplementation change the gut microbiota and prevent the development of hypertension and heart failure in hypertensive miceclinical perspective. Circulation 135, 964–977. doi: 10.1161/CIRCULATIONAHA.116.024545

Organ, C. L., Otsuka, H., Bhushan, S., Wang, Z., Bradley, J., Trivedi, R., et al. (2016). Choline diet and its gut microbe-derived metabolite, trimethylamine n-oxide, exacerbate pressure overload-induced heart failure. Circul. Heart Failure 9, e002314. doi: 10.1161/CIRCHEARTFAILURE.115.002314

Purushe, J., Fouts, D. E., Morrison, M., White, B. A., Mackie, R. I., Coutinho, P. M., et al. (2010). Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii: insights into their environmental niche. Microb. Ecol. 60, 721–729. doi: 10.1007/s00248-010-9692-8

Wang, W., Chen, L., Zhou, R., Wang, X., Song, L., Huang, S., et al. (2014). Increased proportions of Bifidobacterium and the Lactobacillus group and loss of butyrate-producing bacteria in inflammatory bowel disease. J. Clin. Microbiol. 52, 398–406. doi: 10.1128/JCM.01500-13

President Biden tells grads to prepare for a changing world

Cadets tossing caps in airU.S. Air Force Academy Class of 2023 graduates toss their hats into the air after their graduation ceremony at Falcon Stadium June 1, 2023. The commencement speaker was President Joe Biden. (U.S. Air Force Photo by Trevor Cokley)

By Randy Roughton
U.S. Air Force Academy Strategic Communications 

U.S. AIR FORCE ACADEMY, Colo. President Joe Biden told U.S. Air Force Academy Class of 2023 graduates June 1 that they will soon help change the rapidly changing world that awaits them.

The president said the world is at “an inflection point,” and that the new second lieutenants are ready to face the challenge.

“The decisions we make today are going to determine what the world looks like decades from now,” Biden said. “No graduating class gets to choose the world into which they graduate. But a few classes, once every several generations, enter at a point in our history where they actually have a chance to change the trajectory of their country. You’ll face that inflection point today and I know you are going to meet the moment to make sure that the future we’re building is one that fundamentally aligns with our values and protects America’s interests.”

Most diverse class to date

Biden gave the commencement speech for the Class of ‘23 at the Academy’s graduation ceremony in Falcon Stadium, speaking to 921 graduates, faculty, staff and thousands of friends and relatives. He praised the class for its diversity. This year’s class had the highest graduation percentage of women and minorities in the Academy’s history, Biden said. Twenty-nine percent of the class were women, and minorities comprised 31% of the graduates. He noted the Academy achieved this feat during the 75th anniversary of the Women’s Armed Services Integration Act and the 50th anniversary of an all-volunteer military.

The president also encouraged the graduates to use their character and leadership to meet the challenges that await them.

President Biden shaking hands with graduating cadetPresident Joe Biden greets a U.S. Air Force Academy cadet at the Class of 2023 graduation ceremony at Falcon Stadium June 1, 2023. (U.S. Air Force Photo by Justin Pacheco)

“The power of our example”

“I’ve always believed that America is strongest when we lead, not by the example of our power but by the power of our example,” Biden said. “The same can be true of your own experience in leadership. No matter what changes or challenges come, your character, your moral clarity and your capabilities must never waver.”

Before Biden took the podium, Lt. Gen. Richard M. Clark, Academy superintendent, compared the class’s resiliency, particularly their response during the COVID-19 pandemic, to that shown by the officer they chose as their exemplar, Lt. Col. Leo K. Thorsness. Each class selects one Airman who served significantly in the Air Force as an exemplar to follow.

This year’s class exemplar was a Vietnam-era fighter pilot who led a flight of four F-105 Thunderbirds on a mission in North Vietnam April 19, 1967. Despite the extreme danger, Thorsness remained behind when one of his wingmen was shot down. He shot down one MiG-17 and took on four more when he spotted them over the downed aircraft. Thorsness later received the Congressional Medal of Honor for his actions. Eleven days later, he was shot down, captured, and remained a prisoner of war for more than six years.

Thorsness, who died in 2017, “did not falter, and he did not fail,” Clark said, paraphrasing the last two lines in the Airman’s Creed.

Surviving and thriving

“During your time at the Academy, I saw firsthand the hardships the Class of 2023 had and how you reflected the qualities of your exemplar,” Clark said. “Like Leo Thorsness, you did not falter and you did not fail. During your first year, you found yourselves in the middle of a pandemic and we sent you home in March to learn [virtually]. The dean, staff and faculty pivoted on a dime and moved from an in-person curriculum to distance learning.Other universities were forced to shut down, but we couldn’t because your country needed you.”

Clark added, “I’m proud to say that you didn’t just survive; you thrived.”

Cadets hugging at graduationTwo U.S. Air Force Academy Class of 2023 graduates embrace during their graduation ceremony at Falcon Stadium June 1, 2023. President Joe Biden was the commencement speaker. (U.S. Air Force Photo by Justin Pacheco)

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