Migrant bus arrives in Philadelphia as Title 42 ends

A bus carrying immigrants who crossed the border into Texas arrived in Philadelphia early Wednesday, sent by Gov. Greg Abbott as part of his ongoing effort to target northern sanctuary cities.

It was the first bus to land here in four months, coming as local officials readied for more arrivals amid a potential surge of people at the southern border.

The bus rolled onto a street near near 30th Street Station shortly before 6:30 a.m., carrying 31 passengers including seven children.

A smaller-than-usual welcoming party of immigration advocates and city employees prepared to hand out light jackets and juice boxes as people disembarked in chilly temperatures.

”Hi, my friend, good morning,” said one rider to the advocates, wanting to practice his English. Five arrivals were set to go to the airport, with Chicago as their final destination.

What’s next is uncertain, in Philadelphia and on the border — but officials worry the Thursday end of Title 42, a Trump-era pandemic policy that let the government quickly turn away certain migrants, will generate a surge of arrivals and worsen a humanitarian crisis.

President Joe Biden took office promising to create a more humane immigration system, but in fact has continued some Trump policies, including Title 42, a World War II health law allows the government to expel people in the name of stopping communicable diseases.

Now that policy is ending, to be replaced by other federal barriers to entry.

The trip to Philadelphia was not smooth, according to people who disembarked on Wednesday. Some children developed stomach problems from the prepackaged military meals that were provided to them.

All those sent to Philadelphia have legal permission to be in the country, and most or all are seeking asylum, a protection that can be provided to people who could be harmed in their homelands.

Nicole Gomez, 20, originally from Venezuela, said there were miscommunications over where passengers were being sent. She and four other women signed up to be bused to Chicago, but when they climbed aboard the bus in Del Rio, Texas, it was headed to Philadelphia.

Still, she said, she was happy to be in the United States, making her way to Philadelphia International Airport with four other women.

”The economic situation [in Venezuela] forced us to come, and it’s a shame because it’s a beautiful country,” she said. “But I’m ready to seek the necessary paperwork and begin working.”

Another woman, Isamar Granadillo, 33, said she wanted to get an education in this country.

The other 26 migrants headed to the city-run welcome center to arrange pickups and travel to New York and other nearby cities — and to grab a bite of hot food for the first time since Monday afternoon, when the bus departed.

Blanca Pacheco, codirector of New Sanctuary Movement of Philadelphia, said organizers here remain ready to help people like Granadillo and Gomez.

She was critical of President Joe Biden, who promised a more humane immigration system and a pathway to citizenship for newcomers across the country. At the least, she said, the administration should have created a welcome protocol at the border to help asylum-seekers.

The welcome system in Philadelphia has worked, she said, and the federal government should set up the same kind of ordered procedure.

“They should support the asylum seekers, and then make work permits available for them as soon as possible,” she said.

The previous bus from Texas arrived in Philadelphia on Jan. 7. Abbott’s office did not immediately respond to a request for comment early Wednesday.

That winter arrival was the 19th in a string of unscheduled, unannounced buses that began landing shortly before Thanksgiving, carrying nearly a thousand immigrants to this city. Most people have moved on to join family in other states.

On Wednesday advocates estimated that about 11 migrants were staying at the city welcome center.

Abbott called the buses an effort to relieve overwhelmed border towns, while Mayor Jim Kenney and others said it was a cheap political trick that hurt innocent people.

Philadelphia has been targeted among so-called sanctuary cities, including Chicago, New York, and Washington; Abbott sent more than 16,000 migrants in 2022. Mayors in Washington and New York declared states of emergency due to the influx of people.

This month more buses rolled into New York, and Republican leaders of two suburban counties declared states of emergency there, to try to stop the city’s Democratic mayor from housing hundreds of migrants in their communities.

Immigrant advocates in Philadelphia have been preparing for fresh arrivals, with the pandemic-related immigration restrictions of Title 42 scheduled to expire on Thursday.

The Trump administration used Title 42 it to override asylum, a legal means to stay in the United States for those facing persecution in their homelands. The administration sent people who entered the southern border back to their home country or, more often, to Mexico.

The Biden administration continued the policy and defended it in court — and later opposed it in legal proceedings. Now the Centers for Disease Control says the COVID-19 pandemic no longer justifies the use of Title 42.

It might provoke a rush of new arrivals toward such states as Texas, followed by the sending of more buses to Philadelphia from Del Rio, a border town located about 150 miles west of San Antonio.

The first bus on Nov. 16 brought about 30 migrants to a side street near the train station, and established what has since become the routine procedure: passengers were quickly checked for medical emergencies, then offered blankets, coats, and drinks. Some were picked up by family members, while others boarded a waiting bus to be transferred to the city welcome center, where government workers and immigrant-assistance groups provided food, temporary housing, legal services, and logistical support.

During roughly seven weeks, 838 immigrants arrived here, according to city officials. Almost all moved on, connecting with family in surrounding states.

The local impact was real, forcing Philadelphia officials and immigrant leaders to respond to every bus, whatever the day or hour, tiring them out, upending their schedules, and diverting them from other work.

The Biden administration has announced that as Title 42 expires, new rules will take effect, including one that presumes people who did not seek protection in other countries on their way here are ineligible for asylum.

That’s a dramatic reinterpretation of immigration law, which says people can seek protection no matter how they got here. It’s unclear how potential court challenges to that rule could affect its implementation.

The right to seek asylum sprang from the worldwide displacement of WWII and the horror of the Holocaust, enshrined in the 1948 Universal Declaration of Human Rights, in the Refugee Convention of 1951 and in the United States Refugee Act of 1980. Those acts offer safety to people fleeing persecution based on their race, religion, nationality, political opinions, or membership in a particular social group.

Earlier arrivals interviewed by The Inquirer said their two-day bus journey started with an offer of a free trip to Chicago, New York, or Philadelphia, made over a loudspeaker at a Texas border facility. Many had come to the United States from Colombia, the Dominican Republic, Panama, and Cuba.

The city’s Office of Immigrant Affairs and the Office of Emergency Management had spent months preparing for arrivals, coordinating with community organizations including Juntos, HIAS Pennsylvania, the Pennsylvania Immigration and Citizenship Coalition, and Nationalities Service Center.

Trump Media took $8 million loan from porn-tied bank, unknown trust – The Washington Post

Yet the role ES Family Trust would assume in Trump Media and Technology Group has never been officially disclosed to the Securities and Exchange Commission or to shareholders in Digital World Acquisition, the special purpose acquisition company, or SPAC, that has proposed merging with Trump’s company.

The companies also have not disclosed to shareholders or the SEC that Trump Media paid a $240,000 finder’s fee for helping to arrange the $8 million loan deal with ES Family Trust — or that the recipient of that fee was an outside brokerage associated with Patrick Orlando, then Digital World’s CEO.

Messages sent to ES Family Trust’s only known trustee and an associated email account yielded no response. A person who answered a phone number listed for the bank that transmitted the loan, Paxum Bank, said the company declined to comment. Representatives for the bank’s owner, Anton Postolnikov, did not respond to requests for comment.

The ongoing investigation has halted Digital World’s ability to merge with Trump Media and unlock more than $1 billion in investor funds. Digital World’s board fired Orlando as chairman and chief executive in March, saying in an SEC filing that the company was facing “unprecedented headwinds” and that his “departure enables the Board to appoint new leadership, which it believes will restore confidence to the shareholders.” Orlando did not respond to requests for comment.

In a separate SEC filing last month, the company said that, by the end of last year, it had just $989 in cash on hand and more than $17 million in debts. Most SPACs don’t rack up anything close to this level of debt, Ohlrogge said, because “the normal costs of running a SPAC, finding a target and negotiating with that target are not very expensive.”

In late 2021, the frozen merger was also raising concerns inside Trump Media over its ability to pay the bills, Wilkerson recalled. Then, he said, Orlando revealed he had made a breakthrough: a loan deal worth up to $8 million from an entity called ES Family Trust.

In a convertible promissory note dated Dec. 23, 2021, Trump Media was offered the money in exchange for agreeing to “automatically” convert the loan principal into “shares of Company Stock” once the merger with Digital World occurred, according to a copy of the document reviewed by The Post. The document does not say precisely how much stock ES Family Trust would receive. Trump estimated last month that the company, of which he owns 90 percent, is worth between $5 million and $25 million.

In January 2022, Trump Media agreed to pay a cash referral fee — equal to 3 percent of the $8 million loans, or $240,000 — to a Houston-based brokerage firm called Entoro Securities, according to a referral fee agreement and an Entoro invoice provided by Wilkerson.

The referral fee agreement names “Anton Postolnikov and affiliated entities” as “Introduced Parties” who participated in the deal. Orlando is a registered broker at Entoro, according to a database run by Finra, and Orlando’s LinkedIn profile says he has been a managing director there since 2020.

An attorney working with Trump Media, John Haley, sent a brief email about the first $2 million in December 2021 to Donald Trump Jr., before the former president’s son joined the company’s board, saying there was “no guaranty that these will get signed and funded, but we remain hopeful,” according to a copy of the correspondence shared by Wilkerson.

A month after the second payment, Trump Media executives still knew little about the origin of the money. On March 8, 2022, the company’s chief financial officer, Phillip Juhan, sent an email to the then-chief legal officer of another Orlando-run firm, Benessere Investment Group, seeking contact information for anyone at ES Family Trust.

The response from the Benessere executive, Alexander Monje, was a single sentence: “Hey Philip, it is [email protected],” with no other names or addresses attached, according to a copy of the exchange shared by Wilkerson. Proton Mail is an encrypted email service based in Switzerland.

Digital World did not tell investors about the $8 million in loans or Entoro’s referral fee in its filings submitted to the SEC, a review of public documents shows. Ohlrogge said the SEC could insist the loans should have been disclosed to investors, given that the concerns over its origins and Orlando’s finder’s fee could affect the value of the shares.

“The right way for him to have done this is to say: ‘I know this transaction looks potentially bad and may be enriching me at the expense of [Digital World] shareholders, but I really think it’s ultimately in their best interest,’” Ohlrogge said. “That’s the right way to do it. And he didn’t do anything of the sort.”

Postolnikov, whose name appeared on the referral-fee document, is an owner of Paxum Bank and employs Pacheco, ES Family Trust’s named trustee. Postolnikov said in a 2018 federal court filing that he is “the principal of Paxum Bank,” and a company statement in March called him its “primary owner.”

Paxum itself remains a mystery. The company has promoted itself online as a way for video streamers of adult content to coordinate financial transactions across international borders and, in 2021, Paxum’s then-chief executive, Andrei Octav Moise, told BCAMS Magazine, a trade publication for the business of live webcam models, that the bank was “proud and happy to be considered the #1 trusted payment service for the adult industry!”

On Thursday, Moise insisted that he wasn’t familiar with Paxum’s activities or the Trump Media transaction and said he “never had any job or [ownership] or any control” in the bank. On Friday, the adult-industry trade publications AVN and Xbiz reported that Moise had decided to push for a sale of Paxum and would be “retiring” from the industry. He was quoted as saying, “It has been both a privilege and a pleasure to lead the development of Paxum into one of the premier global payment platforms.”

It’s unclear when Postolnikov moved to the United States. In the past two years, he has invested millions of dollars in luxury real estate on Fisher Island, a private enclave off the coast of Miami Beach that Bloomberg in 2020 named “America’s richest Zip code.” Miami-Dade property records show that a company he owns bought two waterfront condos: one for $6 million in April 2021 and another for $7 million in December 2021.

DeSantis representatives did not respond to an email asking whether DeSantis and Postolnikov had a personal or business relationship. The governor filed a notice this week that he is no longer associated with the committee, a standard move due to his expected shift toward a presidential primary campaign in which he would compete against Trump.

Postolnikov and his aunt, Smirnov’s wife, Elena Smirnova, previously worked together at Russia’s United Bureau of Credit History, according to Delovoy Peterburg. There is no evidence that Smirnov or Smirnova are involved in the Trump Media deal.

After the Guardian’s report, an online news outlet in Dominica called Nature Isle News published a response attributed to an unnamed firm representing Paxum and Postolnikov. That response said Postolnikov was a U.S. citizen and had for two years “been the subject of false media smears that have originated in Russia, all to blackmail Anton into paying bitcoin to remove the stories from the internet.”

Carlisle Jno Baptiste, the Nature Isle News’ managing editor, said a Paxum lawyer sent him the response after he asked the company for comment on the Guardian’s report. Baptiste said Paxum has an office in the center of Roseau, the island’s capital, but that it had rarely been a topic of local conversation before the money-laundering claims arose.

Company-SPAC mergers are traditionally quick and straightforward affairs, according to a 2020 presentation by lawyers at the firm Morgan Lewis. A SPAC submits a merger registration document called an S-4; the SEC reviews it and asks further questions, often within 30 days; the company sends in answers in an amended filing, the SEC responds within 14 days, and the whole process generally wraps up within a matter of months.

That delay has become the focus of Trump Media CEO Nunes and other Trump allies, who argue that biased regulators at the SEC have delayed the merger in hopes of suffocating Trump’s company and fueling a political witch hunt.

“The SEC and [SEC Chair Gary] Gensler have been holding this up. The guy clearly has a grudge against us, against Trump,” Nunes said last month on the right-wing news channel Newsmax. “This is a major scandal that’s brewing at the SEC: their willingness to play politics and discriminate against people who like our little company, our start-up company … [and] are being wiped out.”

At a House Financial Services Committee hearing last month, Gensler told Republicans who were grilling him about the delay that SEC officials were working to make sure all merger filings “are accurate and in compliance with the law.”

But Republicans weren’t calmed. The SEC is “focused on a woke political agenda rather than focused on their job,” Rep. Bryan Steil (R-Wis.), chair of the House Administration Committee, which oversees House management matters and U.S. Capitol security, said in a recent interview with John Solomon of the conservative media outlet Just the News.

In October 2021, when Digital World’s merger announcement sparked a stock-market frenzy, Sas bought nearly 1,200 shares at $70. Then, while listening to investing YouTubers predict that the stock could go to $300, he said, he felt a deep fear of missing out, saying, “FOMO hit me hard.”

He entered a market order to buy thousands more shares, expecting he would get them at $90, but the stock’s volatility led to a halt in trading. When the stock came back online, he said, his order was unexpectedly filled at the stock’s all-time high, $175 a share, draining his entire account.

All in all, he spent more than $516,000 in cash on Digital World shares, according to investment account documentation he provided. “This was basically my life savings,” he said, adding that he doesn’t own any property and drives a 2011 Ford Fiesta. Digital World shares now trade for about $13.

Sas said he was initially excited about the prospect of Truth Social being a “true, non-biased news outlet” and that he hopes “the people running Trump Media, if they see some things not going right, they get in there and fix it.”

He echoed other supporters of Digital World and Trump in blaming a “biased” SEC for stalling the merger. “I’m left holding the bag now, hoping something goes through and it doesn’t collapse,” he said.

The Journey of Frank Gabriel Pacheco: From Humble Beginnings to Extraordinary Achievements

The Journey of Frank Gabriel Pacheco: From Humble Beginnings to Extraordinary Achievements

Introduction:

Frank Gabriel Pacheco, an exceptional individual who emerged from humble beginnings, has forged a remarkable path of achievements throughout his life. From his early upbringing in a modest neighborhood to his ascent as a renowned entrepreneur and philanthropist, Pacheco’s journey is one that exemplifies the power of perseverance, dedication, and unwavering belief in oneself. This biography delves into the life story of Frank Gabriel Pacheco, shedding light on the pivotal moments, challenges faced, and extraordinary accomplishments that have defined his legacy.

Chapter 1: Early Life and Challenges

Born on February 10th, 1965, in a small town in New Mexico, Frank Gabriel Pacheco was raised by hardworking parents who instilled in him the values of integrity, resilience, and ambition. Growing up in a close-knit community with limited resources presented its own set of challenges for young Frank. However, it was these very circumstances that fueled his determination to rise above them.

Despite financial constraints and limited educational opportunities within his locality, Pacheco’s thirst for knowledge was insatiable. He displayed exceptional academic prowess from an early age and often found solace in books. With the support of his parents and teachers who recognized his potential, Frank managed to secure scholarships to pursue higher education at prestigious institutions.

Chapter 2: Educational Pursuits and Professional Success

Driven by an unyielding desire to make something significant out of himself, Frank Gabriel Pacheco pursued higher education with fervor. He enrolled at the renowned Harvard University where he pursued a degree in Business Administration. During his time at Harvard, he distinguished himself as an exemplary student who possessed not only intellectual acumen but also remarkable leadership skills.

Upon graduating with honors from Harvard University in 1987, Pacheco embarked on an illustrious career in the corporate world. He joined a prominent investment firm where his astute financial acumen and visionary approach to business propelled him to new heights. Pacheco’s ability to identify lucrative investment opportunities and navigate complex market dynamics garnered him widespread recognition within the industry.

Chapter 3: Entrepreneurship and Innovation

While enjoying a successful career in the financial sector, Frank Gabriel Pacheco harbored an entrepreneurial spirit that burned within him. In 1995, he made the audacious decision to venture into the world of entrepreneurship, founding his own technology start-up. Guided by his innate ability to foresee trends and identify untapped markets, Pacheco’s start-up quickly gained traction and transformed into a global powerhouse.

Under Pacheco’s innovative leadership, his company revolutionized the field of technology with groundbreaking products that disrupted traditional industries. His relentless pursuit of excellence coupled with an unwavering commitment to customer satisfaction propelled his company’s success to unprecedented levels.

Chapter 4: Philanthropy and Humanitarian Contributions

Frank Gabriel Pacheco firmly believes in giving back to society and making a positive impact on people’s lives. Throughout his journey of personal success, he has remained committed to philanthropy and humanitarian causes. His charitable foundation has supported numerous initiatives aimed at empowering underprivileged communities, providing access to education, healthcare, and sustainable livelihoods.

Pacheco’s dedication towards social welfare has earned him accolades globally. He has been recognized as a champion for social justice and equality, actively working towards creating a more inclusive society for all.

Chapter 5: Legacy and Future Endeavors

As Frank Gabriel Pacheco reflects upon his life’s journey, he acknowledges that it is not only about personal accomplishments but also leaving behind a lasting legacy for future generations. With an insatiable appetite for knowledge and an unwavering commitment to excellence, Pacheco continues to explore new avenues for growth and innovation.

In the twilight of his career, Pacheco endeavors to mentor aspiring entrepreneurs, imparting his invaluable wisdom and experiences. He aims to inspire others to overcome obstacles, embrace their unique potential, and create transformative change in their respective fields.

Conclusion:

The life of Frank Gabriel Pacheco is a testament to the power of perseverance, determination, and unwavering belief in oneself. From his humble beginnings to extraordinary achievements, he has transcended societal barriers and emerged as an exemplary figure in the realms of business and philanthropy. Frank Gabriel Pacheco’s journey serves as an inspiration for generations to come – a reminder that with vision, hard work, and unwavering dedication, anyone can overcome adversity and make a lasting impact on the world.

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The Top 5 Fragrances And Perfumes Of All Time

The Top 5 Fragrances And Perfumes Of All Time

Individuals enjoy fragrance. Since individuals like to constantly scent great, fragrance makers constantly attempt to come up with a scent that would certainly scent appealing to individuals.

Via the years, numerous brand names of fragrances have actually been presented to the marketplace. All appear to scent great. There are just some that continue to be in the market as well as have actually been all-time faves by numerous individuals as well as are also put on as well as recommended by some of the widely known and also popular characters in the globe.

The preference for fragrance is various for everyone. In spite of this reality, the preferences for fragrances constantly have a factor where virtually everyone’s preferences appear to satisfy as well as praise. Below are the leading 5 kept in mind fragrances for ladies of all time.

* Chanel No. 5
One of the most well-known characters that assert to like this fragrance much is none various other than Marilyn Monroe. In a meeting, she stated she would not use any kind of fragrance to bed however Chanel No. 5.

* L’heure Bleu
It was developed by Guerlain long prior to various other fragrances were produced. This fragrance has a sweeter scent that develops its wonderful aroma.

* Creed Tuberose Indiana
It was developed in 1980 and also previously, several females consisting of popular stars pursue it. Its fruity as well as citrus aroma that produces an attractive sensation is enjoyed by Madonna, Jennifer Love Hewitt, Goldie Hawn as well as others.

* Bulgary Pour Femme
It is one of the most initial aromas of Bulgari which was produced in 1994. This mix develops a flower aroma which is liked by Whitney Houston, Lisa Kudrow as well as others.

* Christian Dior Eau de Dolce Vita
This citrus pleased as well as flower fragrance was produced by Christian Dior. Understood to anybody, specifically celebs such as Charlize Theron and also Kristin Scott Thomas, this fragrance produced in 1998 produces a charming fragrance of feminineness.
Fragrances are not just developed for ladies. Like ladies’s fragrance, numerous brand names have actually come as well as go yet there are these fragrances that males enjoy the majority of.

* Giorgio Armani Emporio Armani Night
It was released just in 2003, this fragrance has a mix of flavor as well as fragrant timbers and also grapefruit that produces a manly impact has actually risen high in the market. This is best for older and also more youthful males.

* Polo Sport by Ralph Lauren
With its tidy aroma, it is best for males constantly on the move. It was presented in 1978 as well as previously, males still opt for this perpetuity preferred aroma. It’s a traditional fragrance that never ever actually ages.

* Hugo Boss for Men
This scent is excellent for males energetic. The mix of flavors as well as fruits communicates quality bound by a manly result.

* Cool Water Cologne Eau De Toilette Spray for guys by Davidoff
With its trendy fragrance, many males like utilizing this Davidoff produced scent. It was developed in 1988 mixing the finest of sage, coriander and also rosemary highlighted with brownish-yellow as well as oak timber. This finest mix developed a scent ideal for day-to-day usage.

* Eternity Cologne Eau De Toilette Spray for guys by Calvin Klein
Calvin Klein introduced this ideal fragrance for males in 1989. It consists of the different mix of hot as well as wonderful fragrances that produce an awesome manly result to those that utilize it.

The above stated leading 5 fragrances for ladies as well as guys are simply overview to aid you select you’re your selection. Numerous guys as well as females, not to discuss various popular individuals in the globe have actually liked them making them the leading 5 amongst numerous various other brand names.

It was produced by Guerlain long prior to various other fragrances were produced. Fragrances are not just produced for females. Like ladies’s fragrance, numerous brand names have actually come as well as go yet there are these fragrances that males enjoy many. It was released just in 2003, this fragrance has a mix of flavor and also fragrant timbers as well as grapefruit that develops a manly result has actually risen high in the market. The above discussed leading 5 fragrances for females as well as guys are simply overview to aid you choose you’re your selection.

Coimbra Biennial of Contemporary Art presents Anozero’21-22: Meia-Noite

Anozero’21-22
Meia-Noite

April 9–June 26, 2022

Anozero’21–22 Meia-noite (Midnight) gathers works by the artists and collectives: Ana Pi, Aurélia de Souza, Beatriz Santiago Muñoz, Candice Lin & P. Staff, Carlos Bunga, Christian Nyampeta & Obi Okigbo, Daniel Steegmann Mangrané, Diana Policarpo, Elisabetta Benassi, Elise Florenty & Marcel Türkowsky, Em’kal Eyongakpa, Euridice Zaituna LaKala, Filipa César, Gabriel Chaile, Jarbas Lopes, Jesse Darling, Jessica Warboys, Joana Escoval, Julie Béna, Lastenia Canayo—Pecon Quena, Laura Huertas Millán, Laura Lamiel, Louidgi Beltrame, Lygia Pape, Maja Escher, Mané Pacheco, Marinho de Pina, Marta Lança, Mary Beth Edelson, Meris Angioletti, Minia Biabiany, Musa paradisiaca, Nelson Pereira dos Santos, Paul Mpagi Sepuya, Ru Kim, Sarah Maldoror, Seni Awa Camara, Sónia Vaz Borges, Vivian Suter, Yoan Sorin.

The Joanine Library—a pearl of the Baroque and a treasure of the University of Coimbra—was built as an imperialist gesture that aimed to encapsulate knowledge and flaunt colonial dominion. This fortress of knowledge (and power) is also the refuge of a small colony of bats that found there the ideal conditions to set up a house. Under the cover of the nightly silence, the bats prey on the insects and larvae that would otherwise munch through the library’s 55,000 books. The night is their time of freedom, the moment when they come out of their hiding places and start working on the conservation of our shared estate. For us, the fact that there are bats living in the Joanine Library was a spark that triggered a cascade of thoughts. We hope that they can offer the same inspiration to everyone that visits the Biennial.

In our critical idealization of Meia-noite, we imagine the night as a fluid space, a space for breaking norms, open to other visions, knowledges, interactions, and bodies. We envision it as a space of resistance and freedom, a political, metaphysical, and experiential place. Here, the night is a territory for symbiotic relationships between humans and other beings, a place for co-dependency and co-evolution. In this perspective, the night fascinates us with its plural capacity to “transvalue” universal normative categories.

The university is, pre-eminently, a place of knowledge and power. If libraries are the epitome of (a certain protected kind of) knowledge, and bats the embodiment of darkness, here we find them in a relationship of mutual dependence. Both the library and the bat become political subjects. A central idea of our research for Meia-noite was how to question the production of knowledge embodied by the Joanine Library. How can we propose alternative epistemologies? How can we learn from the intelligence of bats? How can we imagine other forms of interaction?

Our intention was to free ourselves from the narratives and normative dualism of modern thought that fracture our contemporary society and produce an imbrication of discriminations: racism, classism, sexism, ageism, ableism, to name just a few. With this in mind, we applied methodologies that help us to experiment with creative, unexpected, and marginal modes of knowledge production. We placed the eyeglasses of these Coimbra bats over our feminist ones and invited a group of artists to share their artistic and critical tools with us. Tools that multiply and connect worlds, that come from some existing place after the patriarchy: tools of the future.

Inspired by the night, this proposal for Anozero’21-22 is an attempt to open a speculative fissure, one that does not propose any answers but raises many questions. It is an attempt to invent other modes of existence, of relating, of being, and living together. —Elfi Turpin & Filipa Oliveira, curators

The program of Anozero Biennial develops the central theme and exhibition into three more areas of dialogue between artists, and between artists, local communities, and visitors: Activation, Publishing, and Converging. The Activation Program promotes conversations between different communities, through mediated activities such as guided tours, workshops, and open classes. Publishing is of high relevance in the Biennial, comprising a catalogue edited in two parts: a first, with texts, dreams, and reflections about Anozero and its artists, and a second, which will consist of a visual essay that documents the works created and presented in Meia-noite. The Converging Program hosts a set of parallel activities proposed by artists and art organizations, such as exhibitions, performances, and other events, and it aims to deepen the research on the central theme by strengthening ties and building new connections with the local production.

Anozero—Coimbra Biennial of Contemporary Art is an initiative of the Círculo de Artes Plásticas de Coimbra. It is organized in conjunction with the Coimbra City Council and the University of Coimbra and was first presented in 2015. Its prime goal is to foster reflection around the circumstances that led to the University of Coimbra—Alta, and Sofia being classified by UNESCO as World Heritage. This fourth edition of the Biennial is one of the highlights of the France-Portugal Season 2022 taking place in Portugal.

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NWCA Announces the 2022 NCAA Women’s Scholar All-America Awards – The Guillotine

Manheim, PA – On Monday, the NWCA announced the full slate of 2021-2022 Scholar All-America Team and Individual honorees for the NCAA Women’s division with awards going to 135 individuals representing 22 institutions.

“On behalf of the NWCA Board of Directors, It is my honor to recognize these exceptional athletes and teams,” NWCA Executive Director Mike Moyer said. “Wrestling takes much focus and sacrifice and we are proud to honor these wrestlers for their obvious commitment to their academic pursuits!”

In the team race, Colorado Mesa University finished atop of the field with a 3.87 GPA followed by McKendree University’s 3.776, and Augsburg University’s 3.766.

For the individual awards, Colorado Mesa led the list of honorees with 13 Scholar All-American selections followed closely by King University’s 12. Adrian, Augsburg, North Central, and Tiffin each had 10 award winners.

TOP 10 SCHOLAR TEAM ALL-AMERICA

RANK – INSTITUTION – GPA
1 – Colorado Mesa University – 3.87
2 – McKendree University – 3.776
3 – Augsburg University – 3.766
4 – Adrian College – 3.765
5 – Augsburg University – 3.727
6 – North Central College – 3.721
7 – King University – 3.711
8 – Tiffin University – 3.647
9 – Lindenwood University – 3.607
10 – East Stroudsburg University – 3.487

2022 NCAA Women’s Individual Scholar All-Americans

Institution Athlete Grade Weight Major
Adrian College Carleigh Czerneski Fr 109 Criminal Justice
Adrian College Angela LoRusso Jr 116 Business
Adrian College Sabine Yonak Fr 130 Criminal Justice
Adrian College Mackenzie Tayerle Sr 130 Criminal Justice
Adrian College Shawna Oesterling So 136 Biology
Adrian College Zoe Nowicki Sr 136 Exercise Science
Adrian College Jasmine Hale So 143 International Business/Japanese Studies
Adrian College Quilaztli Miguel-Lapham Fr 155 Interior Design
Adrian College Megan Vondrasek Jr 155 Exercise Science
Adrian College Nicole Lorenz So 170 Criminal Justice
Albion College Samantha Parcell Fr 123 Mathematics
Augsburg University Emily Shilson Jr 109 Finance
Augsburg University Charlotte Kouyoumtjian Fr 123 Business
Augsburg University Gabby Skidmore Jr 123 Exercise Science
Augsburg University Savannah Vold Sr 130 Exercise Science
Augsburg University Nina Makem Fr 136 Education
Augsburg University Sierra Brown-ton Jr 136 Pre-law
Augsburg University Katerina Lange Fr 143 Business
Augsburg University Kya Rybachek So 143 Education
Augsburg University Kahlea Jolly Jr 155 Education
Augsburg University Destynie Pacheco Jr 155 Psychology
Augustana College Kara Rohlf Fr 130 Undeclared
Augustana College Ruby Gerhardt Fr 130 Biology
Augustana College Abbey Ellis Fr 155 Philosophy/Spanish Education
Chadron State College Kinsey Smith Jr 101 Business
Chadron State College Ryleigh Dye Fr 136 Psychology
Chadron State College Karstin Hollen Fr 143 Business
Chadron State College Jeslyn Jindra Jr 155 Special Education
Chadron State College Kamila Montenegro So 170 Psychology
Colorado Mesa University Kaylie Catalano RSSo 101 Business Administration – Finance
Colorado Mesa University Carisa Epling Fr 109 Exercise Science
Colorado Mesa University Alexis Allen Fr 116 Pre-Radiological Sciences
Colorado Mesa University Lillian Broadrick Fr 123 Exercise Science
Colorado Mesa University Cordey Mallo Fr 123 Business Administration
Colorado Mesa University Marissa Gallegos RSJr 123 Biology
Colorado Mesa University Jamae Barnes RSSo 123 Psychology and Business Administration
Colorado Mesa University Claire DiCugno Fr 130 Accounting
Colorado Mesa University Slate Delsman Fr 130 Psychology
Colorado Mesa University Erica Schroeder Fr 136 Exercise Science
Colorado Mesa University Jolynn Harris RSSo 136 Exercise Science
Colorado Mesa University Lily McKaig Fr 143 Pre-Radiological Sciences
Colorado Mesa University Alex Brulotte Fr 170 Accounting & Criminal Justice
East Stroudsburg University Alanna Barberio Fr 116 Liberal Arts
East Stroudsburg University Emily Klein Fr 123 Nursing
East Stroudsburg University Carmela Cancelmo So 123 Arts & Design
East Stroudsburg University Cameron Nunez So 130 Athletic Training
East Stroudsburg University Avia bibeau So 143 Biology
East Stroudsburg University Rachel Deguiulo So 143 Psychology
East Stroudsburg University shola cascen Jr 191 Business Management
Ferrum College Katrina Anderson Sr 130 Art
Gannon University Katerina Pendergrass So 101 Nursing
Gannon University Julianne Moccia Fr 109 Undecided Science
Gannon University Nikoly Dos Santos Jr 116 Computer Science Software Engineer
Gannon University Caleeann Blarr Sr 123 Applied Exercise Science
Gannon University Anya Knappenberger Fr 130 Physician Assistant
Gannon University Mikayla Dockweiler Sr 136 Cyber Engineering
Gannon University Stephanie Floor RSJr 143 Applied Exercise Science
Gannon University Annabelle Helm Jr 170 Electrical Engineer
Hiram College Katie Vogt-Shields Fr 143 Biology
King University Dianna Holmes Fr 109 Exercise Science
King University Samara Chavez Fr 109 Sport Management
King University Danielle Garcia So 109 Exercise Science
King University Sage Mortimer Fr 116 Undecided
King University Melanie Mendoza So 123 Undecided
King University Cheyenne Sisenstein Sr 123 Criminal Justice
King University Montana Delawder Fr 130 Undecided
King University Phoenix Dubose Sr 130 Psychology
King University Ana Luciano Sr 136 Criminal Justice
King University Ashlynn Ortega Sr 143 Security and Intelligence
King University Viktorya Torres So 155 Undecided
King University Nia Crosdale Sr 191 Business: Accounting and Finance
Limestone University Hannah Mains Fr 136 Health Sciences
Limestone University Kathe Wilken-Yoder So 136 Physical Education
Limestone University Mika Walters Jr 143 BS Psychology
Limestone University Clara Padua Cruz So 143 Business Administration
Limestone University Journey Land Fr 191 Athletic Training
Lindenwood University Valeriia Mamleeva Sr 109 Computer Science
Lindenwood University Amanda McAleavey So 116 Political Science
Lindenwood University Cayden Condit Fr 123 Human Resource
Lindenwood University Emily Ebsen Fr 130 Recreational Therapy
Lindenwood University Jasmine Ward Jr 130 Biology
Lindenwood University Athena Willden Fr 136 Exercise Science
Lindenwood University Julianna Moreno Jr 143 Social Work
Lindenwood University Kaycee Fitero So 191 Exercise Science
Lock Haven University Samantha Reitnour RSFr 101 Psychology
Lock Haven University Madison Packer Fr 109 Secondary Education
Lock Haven University Alayna Gil RSFr 109 Criminal Justice
Lock Haven University Madison Matta RSFr 136 Health Sciences
McKendree University Lizette Rodriguez So 101 Education
McKendree University Natalie Reyna Sr 109 Professional Health and Counseling
McKendree University Aliyah Rollins Jr 116 Psychology
McKendree University Felicity Taylor Sr 116 Education – Mathematics
McKendree University Hannah Hall Fr 123 Business Administration & Entrepreneurship
McKendree University Cameron Guerin Jr 130 Biology
McKendree University Emmily Patneaud So 136 Education
McKendree University Joye Levendusky Sr 170 Exercise Science & Sports Performance
New Jersey City University Izabella Frezzo Fr 109 Psychology
New Jersey City University Victoria Devoto Fr 116 Criminal Justice
New Jersey City University Naomi Henry So 130 Sociology & Anthropology
New Jersey City University Lockslea Mayers So 143 Fire Science
New Jersey City University Sandra Guerrero So 191 Management
North Central College Sydney Petzinger Fr 109 Undeclared
North Central College Angelina Graff Jr 109 Sociology
North Central College Kendra Ryan Fr 116 Exercise Science
North Central College Mateah Roehl So 116 Marketing
North Central College Amani Jones Fr 123 Psychology
North Central College Yele Aycock Fr 130 Exercise Science/Psychology
North Central College Sara Sulejmani Fr 136 Health Science
North Central College Riley Aamold Fr 143 Exercise Science
North Central College Fiona McConnell Fr 170 Health Science
North Central College Yelena Majoyed Jr 170 Psychology
Presbyterian College Ainslie Lane RSSo 116 Political Science
Presbyterian College SierraMarie Miller Fr 130 Biology
Presbyterian College Morgan Norris RSSo 143 Political Science
Presbyterian College Aurelia Santoyo Fr 170 Undeclared
Presbyterian College Hannah McElroy RSJr History with Secondary Education
Schreiner University Alina Kinsey Sr 109 Pre-Law
Schreiner University Jordan Johnston Jr 155 Exercise Science & Fitness Wellness
Schreiner University Symphanie Sampson Fr 170 Pre-Nursing
Tiffin University Sugey Ceja RSJr 116 Psychology: Human Services
Tiffin University Grace Jones Fr 123 Psychology: Human Services
Tiffin University Stephanie Mondragon Sr 123 Psychology: Human Services
Tiffin University Michal Buska Fr 136 Psychology: Human Services
Tiffin University Paige Lenhardt So 143 Criminal Justice
Tiffin University Nia Miranda So 143 Nursing
Tiffin University Aliya Martin So 155 Management: International Business
Tiffin University Taylor Hites Sr 155 Exercise Science
Tiffin University Taryn Martin Fr 170 Psychology: Human Services
Tiffin University Hollie Espinoza Fr 191 Criminal Justice
UW-Stevens Point Brooke Sr 101 Physical Education/Health Promotion
UW-Stevens Point Megan Struble Sr 136 Wildlife Ecology and Management
William Jewell College Emalie Olson So 116 Civil Engineering
William Jewell College Elle Fishburn Fr 123 Nursing
William Jewell College Clare Benton Fr 136 English
William Jewell College Tatyana Turner Fr 136 Political Science

Woman Slain in Gilroy in 1993 by ‘Happy Face Killer’ Identified as Oregon Mother | San Jose Inside

After nearly 30 years, detectives from the Santa Clara County Sheriff’s Office have identified a woman whose body was left on the side of a road in Pacheco Pass east of Gilroy, according to authorities.

Oregon resident Patricia “Patsy” Skiple, who was about age 45 at the time of her death in 1993, was one of at least eight victims murdered by the serial killer known as the “Happy Face Killer,” reported a press release from the sheriff’s office.

Patricia Skiple, 1993 murder victim
Santa Clara County Sheriff’s Office photo

On June 3, 1993, authorities found a female body on the side of Highway 152 in Gilroy. At the time, the woman’s body was dressed in blue clothing and her identity was unknown, deputies said in the release. Cold case detectives later referred to the woman as “Blue Pacheco.”

The Santa Clara County medical examiner’s report had listed the cause of the woman’s death as “undetermined,” authorities said.

The sheriff’s office has continued to investigate the death. Local investigators learned that in 1994, a newspaper in Oregon published a five-part series titled “The Happy Face Serial Killer,” says the press release. An anonymous letter writer claimed to have murdered five people along the West Coast.

Four of the five cases were unsolved at the time, authorities said. The anonymous letter writer was later identified as Keith Hunter Jesperson. He became known as the Happy Face Killer after a smiley face symbol that he signed his letters with.

Authorities have confirmed that Jesperson killed eight female adults over the course of five years, says the press release.

In 2006, Jesperson sent a letter to the Santa Clara County District Attorney’s office, stating that he sexually assaulted and killed an unknown woman along a dirt turnout on Highway 152, authorities said. In 2007, he pleaded guilty to killing “Blue Pacheco,” and was convicted of first-degree homicide.

The female victim was still unidentified at the time of the 2007 murder conviction.

In 2019, sheriff’s cold case detectives revisited the death of Blue Pacheco to follow up on leads, according to the press release. Detectives used “advanced investigative genetic genealogy in an attempt to identify Blue Pacheco.”

The sheriff’s cold case detectives partnered with the DNA Doe Project, a nonprofit organization that provides investigative genealogy services to identify unknown bodies throughout the country. On April 13, detectives identified the 1993 murder victim as Skiple, who was known to family and friends as “Patsy.”

Skiple was a mother who had lived in Colton, Ore. for several years, authorities said.

“Although this criminal case was adjudicated, detectives never gave up as they worked diligently  throughout this investigation to provide closure for the family of Patricia Skiple,” said the press release. “We would like to thank the DNA Doe Project, Oregon State Police Criminal Investigations Division, including Detective Jim O’Connor, and the Calgary Police Service, including Detective Ken Carriere and Analyst Amy Lemieux for their assistance throughout this investigation.”

Maine Central Institute names 3rd quarter honor students – CentralMaine.com

PITTSFIELD — Maine Central Institute has announced the following students were named to its third quarter honor roll for the 2021-22 academic year.

Highest honors

Grade 12: Hyoungjoon An, Hector Bligh, Shuaizhi Sam Chen, Yeji Emily Choi, Yuran Choi, Leah Dechaine, Alice Di Terlizzi, Anna Dospelova, Danielle Dow, Jason Farias, Antonio Ferraro, Jodi A Gahn, Nickolas Caden Humphrey-Stevens, Bohyun Kim, Tommaso Lamon, Eungyun Daniel Lee, Sumin Lee and Ella Drew Louder.

Also, Kristyna Markova, Samantha Martin, Ava McCannell, Gracie Lynn Moore, William Daniel Newschafer, Mi Hoang Kieu Milly Nguyen, Thu Ngan Claire Nguyen, Isabella Eleanor Ortiz, Taylor Kimberly Pronovost, Hyeonjee Elena Rho, Jamie Simeone, Aidan Singh, Elisa Vezzola and Alivia Ward.

Grade 11: Alyssa Zoe Ardry, Jeremy Vincent Collamore, Spencer Franklin Dyer, Jenessa Foster, Wooseong Jang, Tyler Judd, Thongruangsak Manawongsakul and Hannah Ellen Robinson.

Grade 10: Catherine E. Carter, Elizabeth K. Cothran, Camille Dizon, Madison Gillis, Isabelle Layne Grard, Tristan Green, Cihan Guzeltas, Charlotte Rae Hands, Yeseul Ashley Hwang, Hein Nanda Kyaw, Maxine Isabella Pallante, Sungbin Park and Ceara Alyssa Plourde.

Also, Wyatt Vaughan Robinson, Annaleese P. Singh, Noah Speed, Jayden Todaro, Olivia Varney, Addison Kendall Verrill and Kadence Wintle.

Grade 9: Mya Jo Basford, Adrianna Marie Brooks, Serenity Sue Starr Cardenas, Grace Cunningham, Heidi Dechaine, Shuzheng Fu, David Griglio, Hyunji Lim, Jane Mautz, Joseph Parker, Jyllian Riendeau, Celia Ann Stinson, Noah Ward, Jane Weymouth and Dillon Whitney.

High honors:

Grade 12: Seth Ramsey Bagley, Kadin William Braley, Christopher Davis, Sydney Dawes, Alberto Di Girolamo, Katelyn L. French, Kayla V. French, Lucas Mawe Halferty, Ryan Christopher Hansen, Corvidae Irene Hathaway, Lillian Kitchin, Ruby Kitchin, Oliwia Korycka, Megan Linkletter and Charlotte Maria Losticka.

Also, Lillyana Marcello, Kaythean Martinez, Filipa Leonor Gomes Simoes Vaz Martins, Jillian Michaud, Sofia Nocito, Jazlynn Pacheco, Jordan William Plamondon, Lydia-Mae Johna Roderick, Emil Roedland, Kristina Rojkovicova, Natalie Sites, Krista Nohemi Tesen Barrios, Quoc Phu Truong and Lillian Janeane Verrill.

Grade 11: Robin Albert, Ella Bernier, Wyatt DeGrasse, Henry Alessandro Galvez Lopez, Paityn Gould, Julien Graff, Kyle Hall, Valentina Kuttenreich, Mateusz Lojewski, Petar Mikovic, Jeonghyun Min, Hyeonho Moon, Manzi Marcus Mugangala and Riley Nelson.

Also, Gabriel Penteado Manente, Sofia Pescador Lopez, Hailey Marie Pooler, Hannah Rodriguez, Evan Rowell, Olivia Smith, Vo Duc Hieu Tran and Marta Dulce Weinstein.

Grade 10: Mehmet Berke Baran, Skyla Dean, Hudson Erold Holmstrom, Lillian Hunt, Isaac Keresey, Minhyeok Kim, Sebastian Luna Moleon, Heather Nelson, Mason Francis O’Brien, Gabriele N. Ouellette, Irene Parrondo Chiquero, Ethan Mark Peasley, Julia Perez Moreno, Daniel Pierce Jr., Brady Rogers, Andrew Michael Ross and Landon Grant Ross.

Grade 9: Matthew J. Bernier, Apollo Brown, Sophia Hanscombe, Keith William Kennedy, Oscar Keresey, Koda Daniel Knox, Rowan Nelson, Hyewon No, Sebastien J. Rail, Mason Richards, Keydaliz Rivera, Drew Carl Shorey, Libby Elaine Trask and Lily Weymouth.

A systematic review and functional bioinformatics analysis of genes associated with Crohn’s disease identify more than 120 related genes | BMC Genomics | Full Text

Classification and identification of CD genes

The PRISMA flow chart for the selection of studies and genes is shown in Fig. 1. The PRISMA checklist is provided as supplementary information (File S1). The PubMed search imported into PubTerm identified 2496 articles, which referred to 1172 genes. The genes were reduced to 1055 after filtering for human genes (Fig. 1, Table S1). Then, each gene was carefully curated, annotated, and categorized as described in methods. The curation revealed that 400 genes were somehow potentially related to CD while 655 genes were not related due to annotations errors, the gene mention was casual, no association was found, or no evidence of variation was shown (mainly in subsequent gene expression changes). From the 400 genes, 81 were finally categorized as associated with a related disease such as IBD in general, UC, familial diarrhea syndrome, colorectal cancer, or chronic lymphocytic leukemia. Ninety-three genes were classified to other genetic alterations because its gene was uncertain, which included genes identified through haplotypes or intergenic regions in GWAS. Thus 226 genes were confirmed as associated with CD from this curation (Fig. 1).

Fig. 1
figure 1

Summary of categorized genes for Crohn’s disease. The numbers at left in arrows at the bottom represent the genes from search [1], while the numbers at right correspond to search [2]. *SNX20 was added manually

Summary of categorized genes for Crohn’s disease. The numbers at left in arrows at the bottom represent the genes from search [1], while the numbers at right correspond to search [2]. *SNX20 was added manually

We also used GWAS Catalog [30] as a source of gene information. From 22 publications for CD risk, we obtained the 525 comprising variants. Variants were further filtered by removing those whose tagged gene was not reported, were not significant, or the gene or variant was duplicated, leaving only 133 genes (Table S2). From these, 77 were already categorized in the PubMed curation described above. Thus 56 genes were added to our list of genes, 27 intergenic variants were assigned to other genetic alterations, and 29 to GWAS evidence within gene. A list of the considered PubMed abstracts is shown in Supplementary Information (files S2 and S3). In summary, we identified 256 genes associated with diverse aspects of CD (Fig. 1).

A total of 126 genes were found to have experimental evidence of variants in CD. The top 26 genes of this category mentioned in more than 15 abstracts are shown in Table 1, while the genes with less than 15 abstracts are summarized in Table 2 and detailed in the Supplementary Information (Table S4). The topmost frequent genes for this category are well-known for their association with CD [11, 12], such as NOD2, TNF, IL23R, ATG16L1, TLR4, IL10, SLC22A4, SLC22A5, and IRGM (Table 1).

Table 1 The subset of top genes with experimental variants associated with CD (abstracts > 15)

Besides the above 126 genes (Tables 1 and 2), we also found 71 genes associated with CD that were categorized as GWAS evidence within gene where an SNP is located within genomic coordinates, either an intron or exon (Table 3). Additionally, 18 genes were found to be specifically associated with treatment response in CD and 41 related to disease complications (Table 3).

Table 3 Other genes associated with CD for diverse categories. * Retrieved from GWASCatalog. + Retrieved from a panel

Location of the functional variants

Of the 126 genes corresponding to the categories of experimental evidence of variants plus 71 genes with GWAS evidence within gene, only 17 genes (< 12%) were found to be annotated in ClinVar [32]. In this context, to support our systematic categorization, a supplementary file is provided with the information referring to the location of the variants that were not found in ClinVar (Tables S4 and S5). This information was revised and obtained either by the original paper or by the information related to the SNP reported at dbSNP [43].

In-silico functional analysis

To provide an overview of the 126 genes with experimental evidence to CD, a functional bioinformatics analysis was performed [33, 44]. For this, we assessed whether the genes prioritized in our study are indeed statistical and biologically relevant. We performed gene set enrichment analysis in multiple databases containing different biological terms, including pathways (KEGG), diseases (GAD), and gene ontology (GO) terms. Detailed results of all enriched gene sets are present in the Supplementary Information. Because the number of significant terms was high, repetitive, and difficult to interpret, we grouped the terms by biological and genetic similarity (see Methods).

Regarding diseases, as expected, the most similar term to CD is IBD and UC, validating our strategy (Fig. 2). We observed a dense group of genes and diseases where CD is located close to other groups of autoimmune and inflammatory diseases, certain types of cancer, hypersensitivity disorders caused by allergies and intolerance, infections by virus and bacteria, pregnancy complications, and metabolic complications.

Fig. 2
figure 2

Functional and expression analysis of CD-associated Genes. AThe y-axis comprises the GO, KEGG, and disease terms. The x-axis comprises the 126 genes used for the analysis. The heat map show values from 0 to 1, corresponding to the average presence of a gene within all terms merged in each group. Diseases include 10 groups, autoimmune (Lupus Erythematosus Systemic (LES), Psoriasis (PS), Diabetes type 1 (DT1), Vitiligo and Arthritis Rheumatoid (RA), Chronic/Inflammatory diseases (Psoriasis, Endometriosis, Sarcoidosis, and Cystic fibrosis), infections (Leprosy, Tuberculosis, Sepsis, Dengue, Hepatitis, and HIV), cancer (Meningioma, cervical, lung, esophageal, liver, ovarian, stomach and prostate cancer), hypersensitivity (Asthma, Atopy, Celiac disease, and dermatitis), pregnancy complications (Abortion, preeclampsia and premature birth), vascular diseases (Atherosclerosis, restenosis, and thromboembolism), brain and mental diseases (Depression, Migraine, Parkinson and Schizophrenia) and metabolic complications (Hypercholesterolemia, Obesity, Diabetes type 2 (DT2) and metabolic syndrome). B Differential Gene Expression of Genes represented by fold changes of all the CD genes, which show to be significantly different in at least one comparison. * denotes significance at q < 0.1 (holm p-value adjusted). Figures were mainly rendered in R software (https://cran.r-project.org/)

Functional and expression analysis of CD-associated Genes. AThe y-axis comprises the GO, KEGG, and disease terms. The x-axis comprises the 126 genes used for the analysis. The heat map show values from 0 to 1, corresponding to the average presence of a gene within all terms merged in each group. Diseases include 10 groups, autoimmune (Lupus Erythematosus Systemic (LES), Psoriasis (PS), Diabetes type 1 (DT1), Vitiligo and Arthritis Rheumatoid (RA), Chronic/Inflammatory diseases (Psoriasis, Endometriosis, Sarcoidosis, and Cystic fibrosis), infections (Leprosy, Tuberculosis, Sepsis, Dengue, Hepatitis, and HIV), cancer (Meningioma, cervical, lung, esophageal, liver, ovarian, stomach and prostate cancer), hypersensitivity (Asthma, Atopy, Celiac disease, and dermatitis), pregnancy complications (Abortion, preeclampsia and premature birth), vascular diseases (Atherosclerosis, restenosis, and thromboembolism), brain and mental diseases (Depression, Migraine, Parkinson and Schizophrenia) and metabolic complications (Hypercholesterolemia, Obesity, Diabetes type 2 (DT2) and metabolic syndrome). B Differential Gene Expression of Genes represented by fold changes of all the CD genes, which show to be significantly different in at least one comparison. * denotes significance at q < 0.1 (holm p-value adjusted). Figures were mainly rendered in R software (https://cran.r-project.org/)

Gene sets from GO are divided into cellular components, biological processes, and molecular functions. Within biological processes terms, we observed significant enrichment in processes related to response to bacteria, positive regulation of nitric oxide (NO), ERK, and NFκβ, apoptosis, cell proliferation, response to lipopolysaccharide, inflammatory and immune response. For molecular function, cytokine activity, interleukine-1 receptor binding, receptor activity, and protein homodimerization activity were significantly enriched by the CD-risk genes analyzed. For cellular components, only membrane, plasma membrane, and extracellular region were significant. Overall the significantly enriched GO terms point to known CD terms such as the immune response, cytokine activity, and signaling receptors as the primary source of functional causes (in terms as Immune Response, Infection, Interleukin-1 receptor binding, Cytokine and defense response, Cytokine activity, Autoimmune Disease).

Additionally, among the enriched pathways identified were JAK-STAT signaling pathway, cytokine receptor interaction, NOD-like receptor signaling pathway, NF-kappa, TNF signaling, Toll-like receptor (TLR) signaling, T cell receptor signaling, and Osteoclast differentiation. These signaling pathways converge on the activation of NF-κB, a protein complex that controls the transcription of DNA cytokine production and cell survival [45]. In addition, the KEGG comorbidities identified are infectious diseases caused by bacteria, protozoa and virus, and IBD, which are reliable associations due to the relationship between microbes and CD [46]. The mapping is, therefore, an excellent guide to connect genes and important biological aspects of CD (Fig. 2).

Among the genes present in the enriched biological terms, we noted two distinct groups depending on the frequency of their presence in the gene sets and their number of abstracts found by PubTerm, designated as common and sporadic (Fig. 2). Briefly, the 34 common genes are highly related to diseases and biological terms and well-studied. In comparison, the 92 sporadic genes are associated with particular diseases or biological terms and not as studied in CD as the common genes. Among the common group, the most shared genes across concepts are NOD2, TNF, ICAM1 NFKBIA, NFKB1, TNFRSF1A, CD14, ACE TLR4, and TLR9, which are involved in both TNF and of NF-κβ signaling pathways [47,48,49]. Also, IFNG, IL1B, IL6, IL23R, IL10, IL4, IL12B, IL1RN, IL18, and IL4R, which are all well-known cytokines or related genes, and contribute to the inflammatory response and cytokine interaction process [50,51,52]. The sporadic group comprised 92 genes that were much less frequent among enriched terms, where ATG16L1, SLC22A4, IRGM, SLC22A5, TNFSF15, NOD1, PTPN2, PTPN22, and DLC5 being more frequently mentioned.

DNAH12, ERAP2, FUT2, ORMDL3 and, TRAIP were more specifically enriched in the CD disease term and less common for the remaining terms.

Once we noted two clear sets of common and sporadic genes that were associated with specific terms, we considered whether the genes might also be grouped by other phenotypes that could explain CD symptoms. Thus, we used the Gene Network tool, which clusters genes with similar Human Phenotype Ontology. Five sub-networks were identified from the 126 genes categorized as experimental evidence. We then merged three highly interconnected sub-networks and subsequently analyzed the three resultant modules (73, 26, and 26 genes respectively for Module 1 in green, Module 2 in purple, and Module 3 in blue, as shown in Fig. 3). Next, for each module, the genes were also functionally analyzed to identify their potential phenotypic consequences. For this, we also used the Gene Network tool. Only exclusive terms for each module that were significant after Bonferroni correction were analyzed (see Methods).

Fig. 3
figure 3

Network analysis for the three main groups identified. Group 1 (green): 73 genes, Group 2 (purple): 26 genes and Group 3 (blue): 27 genes. Figure adapted from Gene Network tool

Network analysis for the three main groups identified. Group 1 (green): 73 genes, Group 2 (purple): 26 genes and Group 3 (blue): 27 genes. Figure adapted from Gene Network tool

For the first module (green), 60 phenotypes were retrieved; most of them related to severe symptoms such as Ocular complications, Altered immune system, Sepsis, Bowel incontinence, Heart complications, Endocrine abnormalities, Dysphagia and constipation, and muscle problems. For the second module (purple), 11 terms were identified mainly related to Neoplasm of the gastrointestinal tract, Hyperhidrosis, Respiratory complications, and Visual impairment. These symptoms are among common abnormalities detected in IBD patients [53,54,55]. Finally, for the third module (blue), only four consequences were identified, abnormality of glutamine metabolism, abnormality of the small intestine, and two remaining terms related to the facial skeleton. The information related to the module assigned to each gene is provided in Table S3.

Gene expression analysis

A recent gene expression analysis of CD, UC, and controls in the colon and ileum showed that 1008 genes were differentially expressed [56]. We, therefore, explored whether the genes found genetically associated with CD in our systematic review were related to those differentially expressed (DE) between CD or UC relative to their normal ileum and colon gene expression. From the 126 genes, 67 genes were found to be DE for both UC and CD (Fig. 2B). The overlap between the 67 genes with those 1008 is highly significant (p = 1− 290, hypergeometric test), suggesting that our 126 genes are particularly enriched in DE genes. Except for SLC39A8, FAS, IRF5, HSPA1L, and PTEN, the vast majority were indeed more significantly associated with UC than with CD. Moreover, the majority of the genes were less expressed in CD relative to controls. We noted two solute carriers more expressed in CD than in normal colon or ileum; SLC22A4 (ergothioneine, carnitine, tetraethylammonium) probably for detoxification, and SLC22A5 (carnitine), whose variants are reported to affect the function of carnitine and organic cation transporters [57]. There are also two less expressed solute carriers, SLC11A1, with a role in the susceptibility of humans and animals to several infections [58] and SLC39A8, associated with gut microbiome composition [59].

Drug-gene interaction analysis

The main therapeutic drugs for CD are azathioprine [60], infliximab, adalimumab, certolizumab pegol, ustekinumab, vedolizumab [61,62,63], prednisone, hydrocortisone, and hydrocortisone acetate [61]. To explore drug-gene interactions, an analysis was performed in DGIdb [41] using these drugs. There is a total of 78 genes that had a reported interaction with these CD therapeutic drugs, 10 of which were identified in our systematic review (Table 4). We reasoned that focusing on drugs that target the gene variants associated with IBD could be a strategy for CD drug repurposing [64]. Thus, to search for alternative drugs for CD, we used 13 other drugs commonly employed in the treatment of chronic autoimmune and inflammatory diseases [64, 65]. We identified 13 CD genes (IL1B, IL1RN, IL6, ABCB1, XIAP, IFNG, ICAM1, NLRP3, JAK2, PPARG, PTGS2, APOE, and SMAD3) that show some interaction and therefore could be further explored as possible treatments for CD (Table 4).

Table 4 Interactions between therapeutic drugs and the 126 genes

To compare the generated list of genes with genetic panels already in use, we benchmarked within those panels in the GTR [42]. We found 21 panels, of which 19 were specific to Crohn’s disease containing only 2 genes, NOD2 and IL6. The two remaining tests were not specific for Crohn’s, IBD, and related diseases. These tests considered 70 genes, of which 22 were identified as functional variants for CD in our curation (including NOD2 and IL6). Thus, from the 256 genes we found (Tables 3 and S3), 225 genes were not included in any panels for CD or IBD-related disorders.

We also verified the identifications at the Open Targets (OT) platform, whose pipeline includes a fine mapping of variants [31]. Filtering 3093 genes for CD for genetic association score > 0.8, 178 genes were identified. Of these, 3 genes (SNN, SH2B3, and SKAP2) were not identified in the set of 1092 curated genes. From the 126 genes identified here having experimental evidence, 39 showed a low genetic association score for CD (0.5 to 0.79), 8 genes showed a good score (0.8 to 1.0) for IBD, and 52 genes do not have genetic data information in OT for CD nor IBD. Some of the 52 genes show variations that have not been reported in GWAS, explaining their absence in OT. Examples include NOD1, ABCB1, IL1RN, MEFV, and IL18 having variants that could not be easily identified in GWAS because there are triallelic changes [66], deletions [67], and VNTR [68]. This comparison shows that even GWAS information can leave aside some information of other variants detected through other technologies or methodologies.

CCC TOURNAMENT: Pacheco Delivers Walk-Off Double In Extra Innings Comeback Over Nichols – Endicott Athletics and Recreation

BEVERLY, Mass. — Dylan Pacheco (Dartmouth, Mass.) laced a walk-off double to right field to help the No. 4/6 nationally ranked Gulls complete a 3-2 comeback victory against Nichols in 12 innings on Thursday evening at North Field.

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